MmuEX0015311 @ mm10
Exon Skipping
Gene
ENSMUSG00000040265 | Dnm3
Description
dynamin 3 [Source:MGI Symbol;Acc:MGI:1341299]
Coordinates
chr1:162231654-162274539:-
Coord C1 exon
chr1:162274488-162274539
Coord A exon
chr1:162273604-162273649
Coord C2 exon
chr1:162231654-162231767
Length
46 bp
Sequences
Splice sites
3' ss Seq
ATAATTCTTTGCTATTTCAGGGA
3' ss Score
8.14
5' ss Seq
TCTGTAAGC
5' ss Score
4.33
Exon sequences
Seq C1 exon
TGCTCAGCAGAGGAGCAGTCAGGTTCACAAGAAAAGCACAATTGGAAATCAG
Seq A exon
GGAACCAATCTTCCGCCTTCACGGCAAATTGTACGAGCTAAGTTCT
Seq C2 exon
GTGATTCGTAAGGGCTGGCTGACCGTCAGCAACATTGGCATCATGAAAGGGGGCTCGAAGGGCTACTGGTTTGTCCTCACTGCAGAAAGCCTGTCCTGGTATAAAGACGATGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000040265-'27-27,'27-25,29-27
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
In the CDS, with uncertain impact
No structure available
Features
Disorder rate (Iupred):
C1=0.153 A=0.250 C2=0.000
Domain overlap (PFAM):
C1:
PF0103115=Dynamin_M=PD(4.1=66.7)
A:
NA
C2:
PF0016924=PH=PU(35.6=97.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGAGGAGCAGTCAGGTTCACA
R:
GGCTTTCTGCAGTGAGGACAA
Band lengths:
135-181
Functional annotations
There are 2 annotated functions for this event
PMID: 9725914
This event
The authors generated stably transfected Clone 9 cells expressing full-length cDNAs of six different spliced forms of the three dynamin genes (Dnm1, Dnm2, and Dnm3) tagged with green fluorescent protein. The investigated splice variants are: Dnm1 (with mutually exclusive exon 10a (MmuEX6100535) or exon 10b (MmuEX0015279), Dnm2 (with/without cassette exon (MmuEX0015304), and Dnm3 (with/without cassette exon MmuEX0015311). Confocal or fluorescence microscopy of these transfected cells revealed that many of the dynamin proteins associate with distinct membrane compartments, which include clathrin-coated pits at the plasma membrane and the Golgi apparatus, and several undefined vesicle populations.
PMID: 15741233
This event
Dyn3baa (exon inclusion) promotes the formation of immature dendritic filopodia in cultured neurons. This effect is dependent upon Dyn3 GTPase activity and a direct interaction with the F-actin-binding protein cortactin. Consistent with these findings, Dyn3baa binds to cortactin with a 200% higher affinity than Dyn3aaa, a near identical isoform that does not induce dendritic filopodia when expressed in cultured neurons.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types