GgaEX0004120 @ galGal4
Exon Skipping
Gene
ENSGALG00000003164 | DNM3
Description
dynamin 3 [Source:HGNC Symbol;Acc:HGNC:29125]
Coordinates
chr8:4631490-4638770:-
Coord C1 exon
chr8:4638722-4638770
Coord A exon
chr8:4637037-4637066
Coord C2 exon
chr8:4631490-4631603
Length
30 bp
Sequences
Splice sites
3' ss Seq
TTTGCAATTTGCTATTTCAGGGA
3' ss Score
8.01
5' ss Seq
ATTGTACGA
5' ss Score
0.93
Exon sequences
Seq C1 exon
TGCCCAACAAAGAAGCAGTCAGGTTAACAAAAGTGCAGTGGGAAATCAG
Seq A exon
GGAACCAATCTACCGCCTTCAAGGCAAATT
Seq C2 exon
GTCATCCGGAAGGGCTGGCTGACCATCAACAATATTGGCATCATGAAAGGAGGATCCAAAGAATACTGGTTCGTTCTAACTGCAGAAAGCCTGTCCTGGTATAAAGATGACGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000003164_MULTIEX2-2/2=1-C2
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.081 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0103115=Dynamin_M=PD(4.1=70.6)
A:
NO
C2:
PF0016924=PH=PU(35.6=92.5)
Main Inclusion Isoform:
ENSGALT00000005010fB456
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGGTTAACAAAAGTGCAGTGGGA
R:
AGGCTTTCTGCAGTTAGAACGA
Band lengths:
121-151
Functional annotations
There are 2 annotated functions for this event
PMID: 9725914
The authors generated stably transfected Clone 9 cells expressing full-length cDNAs of six different spliced forms of the three dynamin genes (Dnm1, Dnm2, and Dnm3) tagged with green fluorescent protein. The investigated splice variants are: Dnm1 (with mutually exclusive exon 10a (MmuEX6100535) or exon 10b (MmuEX0015279), Dnm2 (with/without cassette exon (MmuEX0015304), and Dnm3 (with/without cassette exon MmuEX0015311). Confocal or fluorescence microscopy of these transfected cells revealed that many of the dynamin proteins associate with distinct membrane compartments, which include clathrin-coated pits at the plasma membrane and the Golgi apparatus, and several undefined vesicle populations.
PMID: 15741233
Dyn3baa (exon inclusion) promotes the formation of immature dendritic filopodia in cultured neurons. This effect is dependent upon Dyn3 GTPase activity and a direct interaction with the F-actin-binding protein cortactin. Consistent with these findings, Dyn3baa binds to cortactin with a 200% higher affinity than Dyn3aaa, a near identical isoform that does not induce dendritic filopodia when expressed in cultured neurons.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]