MmuEX0016412 @ mm10
Exon Skipping
Gene
ENSMUSG00000063600 | Egfem1
Description
EGF-like and EMI domain containing 1 [Source:MGI Symbol;Acc:MGI:1922990]
Coordinates
chr3:29648217-29657267:+
Coord C1 exon
chr3:29648217-29648336
Coord A exon
chr3:29650769-29650891
Coord C2 exon
chr3:29657130-29657267
Length
123 bp
Sequences
Splice sites
3' ss Seq
GTCTCTCTCTCTCTCTGTAGATA
3' ss Score
13.33
5' ss Seq
ACAGTAAGT
5' ss Score
9.49
Exon sequences
Seq C1 exon
AGACAGACCCCTGTGCAGGTGCAAATGGATGTGCTCACCTATGTCAAACTGAAAATGGCATGGCCAGGTGTGCCTGCCACGCTGGGTACCAGCTGTCTGAAGACAAAAAGGCCTGCGAAG
Seq A exon
ATATAAATGAGTGTGCTGAAGAACTCGCTCCCTGTGCTCATCACTGTGTGAACTCAAAGGGGTCCTTCACATGTACCTGCCACCCTGGCTTTGAGCTAGGAGCTGACAGAAAACATTGTTACA
Seq C2 exon
GAATTGAATTAGAAATTGTCAATATCTGTGAGAAGAACAATGGCGGCTGTTCTCATCACTGCGAGCCTGCAATTGGTGGAGCCCACTGTTCCTGTAACCACGGACATCAACTGGATACCGATGGGAAAACATGTATAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000063600-'19-27,'19-25,20-27
Average complexity
C1
Mappability confidence:
89%=75=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF146701=FXa_inhibition=WD(100=85.4),PF0764510=EGF_CA=PU(0.1=0.0)
A:
PF0764510=EGF_CA=WD(100=95.2)
C2:
PF146701=FXa_inhibition=WD(100=76.6),PF0764510=EGF_CA=PU(0.1=0.0)
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCAGGTGCAAATGGATGTGCT
R:
ATACATGTTTTCCCATCGGTATCCA
Band lengths:
242-365
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types