Special

MmuEX0016417 @ mm9

Exon Skipping

Gene
ENSMUSG00000063600 | Egfem1
Description
EGF-like and EMI domain containing 1 [Source:MGI Symbol;Acc:MGI:1922990]
Coordinates
chr3:29556052-29567360:+
Coord C1 exon
chr3:29556052-29556189
Coord A exon
chr3:29561299-29561421
Coord C2 exon
chr3:29567163-29567360
Length
123 bp
Sequences
Splice sites
3' ss Seq
AAATGTCCTCTGCCCTTCAGACT
3' ss Score
6.44
5' ss Seq
ATGGTAAAT
5' ss Score
6.41
Exon sequences
Seq C1 exon
GAATTGAATTAGAAATTGTCAATATCTGTGAGAAGAACAATGGCGGCTGTTCTCATCACTGCGAGCCTGCAATTGGTGGAGCCCACTGTTCCTGTAACCACGGACATCAACTGGATACCGATGGGAAAACATGTATAG
Seq A exon
ACTTTGATGAATGTGAGAGCGGAGAAGCCTGCTGTGCCCAACTCTGCATCAACTACTTAGGAGGCTATGAGTGCAGCTGTGAGGAAGGCTTTCAGATCAGTTCGGATGGTTGTGGATGTGATG
Seq C2 exon
CTTTAGATGAGCAGTTAGAGGAAGAAGAAGAGGAAATAGACATTCTGAGGTTTCCAGGCCGTCTGGCTCAGAACCCACCTCAGCCATTCCCTTATCTTGATCCTTCTCTGACTGCTTCATATGAAGATGAAGACAATGATGATGCTGACTCTGAAGCAGAAGGAGAGGTCCAAGGGCTGACAGCCTTGTACAGAGTTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000063600_CASSETTE2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
Alternative protein isoforms (Ref)





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.000 A=0.000 C2=0.497
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF146701=FXa_inhibition=WD(100=76.6),PF0764510=EGF_CA=PU(0.1=0.0)

							
A:
PF0764510=EGF_CA=WD(100=95.2)

							
C2:
NO

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000112943





     
                   









    
Main Skipping Isoform:
ENSMUSP00000117016





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000096772, ENSMUSP00000112907, ENSMUSP00000112943f5283A
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
No conservation detected
Human
(hg19)
chr3:168525317-168525439 
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
chr1:99433769-99433891 
Chicken
(galGal4)
chr9:19826187-19826309 
ALTERNATIVE
GgaEX0013962
([1])
Chicken
(galGal3)
chr9:21904947-21905069 
ALTERNATIVE
GgaEX0013962
([2])
Zebrafish
(danRer10)
chr23:21237570-21237692 
HIGH PSI
DreEX6080162
(megf6a)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
TGGAGCCCACTGTTCCTGTAA

				
R: 
ACTCTGTACAAGGCTGTCAGC

				
Band lengths: 
258-381

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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