Special

MmuEX0030357 @ mm9

Exon Skipping

Gene
ENSMUSG00000020908 | Myh3
Description
myosin, heavy polypeptide 3, skeletal muscle, embryonic [Source:MGI Symbol;Acc:MGI:1339709]
Coordinates
chr11:66913102-66914470:+
Coord C1 exon
chr11:66913102-66913305
Coord A exon
chr11:66913378-66913503
Coord C2 exon
chr11:66914300-66914470
Length
126 bp
Sequences
Splice sites
3' ss Seq
CACTCCCTCCTCCTCCCCAGAAC
3' ss Score
9.19
5' ss Seq
GACGTGAGG
5' ss Score
4.68
Exon sequences
Seq C1 exon
GACACCCAGCTGCACCTGGATGACGCTCTCCGGGGCCAGGAGGACCTGAAGGAGCAGCTGGCCATCGTGGAACGCAGAGCCAACCTCCTGCAGGCTGAGGTGGAGGAGCTGCGGGCCACGCTGGAGCAGACGGAGAGGGCCCGGAAGCTGGCAGAGCAGGAGCTGTTGGATTCGAATGAGAGGGTCCAGCTGCTGCACACCCAG
Seq A exon
AACACAAGCCTCATCCACACCAAGAAGAAGTTGGAGACTGACCTCACGCAGCTCCAGAGTGAGGTGGAGGATGCCTGCAGGGACGCAAGGAATGCAGAGGAGAAGGCCAAAAAGGCCATCACTGAC
Seq C2 exon
GCCGCCATGATGGCGGAGGAGCTGAAGAAGGAGCAGGACACCAGCGCCCACCTGGAGAGGATGAAGAAGAACCTGGAGCAGACGGTGAAGGACCTGCAGCACCGTCTGGATGAGGCTGAGCAGCTGGCGCTGAAGGGCGGCAAGAAGCAGATTCAGAAACTGGAGACACGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000020908-'38-38,'38-37,39-38
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact





	

	
Alternative protein isoforms (Ref)





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.426 A=0.738 C2=0.684
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0157614=Myosin_tail_1=FE(7.8=100)

							
A:
PF0157614=Myosin_tail_1=FE(4.8=100)

							
C2:
PF0157614=Myosin_tail_1=FE(6.5=100)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000007301





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000104329, ENSMUSP00000131883
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr17:10631611-10631736 
HIGH PSI
HsaEX6021100
(MYH3)
Human
(hg19)
chr17:10534928-10535053 
HIGH PSI
HsaEX6021100
(MYH3)
Rat
(rn6)
chr10:53642827-53642952 
HIGH PSI
RnoEX6047760
(Myh3)
Cow
(bosTau6)
chr19:30232704-30232829 
Chicken
(galGal4)
chr18:540236-540361 
Chicken
(galGal3)
chr18:594901-595026 
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
GAGGACCTGAAGGAGCAGCT

				
R: 
CCGTCTGCTCCAGGTTCTTCT

				
Band lengths: 
250-376

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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