Special

MmuEX0032069 @ mm9

Exon Skipping

Gene
ENSMUSG00000015468 | Notch4
Description
Notch gene homolog 4 (Drosophila) [Source:MGI Symbol;Acc:MGI:107471]
Coordinates
chr17:34701213-34702713:+
Coord C1 exon
chr17:34701213-34701454
Coord A exon
chr17:34702246-34702327
Coord C2 exon
chr17:34702418-34702713
Length
82 bp
Sequences
Splice sites
3' ss Seq
TCTTCCAGTCTCTTCTCCAGAGC
3' ss Score
6.24
5' ss Seq
CCAGTGAGT
5' ss Score
8.28
Exon sequences
Seq C1 exon
AGTAGGGTGTCCAGGACATTGTGTGACTCAGGAAACAGCTCAGACTGAGGCGTGCAGCAGGCTCAGGAGGAAGAAGGGCGGTAGAGGCAGAGGAGGTGGCCTTGCCTGGCCACAAGGGCTCTGAGGGTCCCTGCCTGAAGAGGGAGAGGAGATCCGGGCCAGGGGCAGGCGCTCTGGAATGCAGCCCCAGTTGCTGCTGCTGCTGCTCTTGCCACTCAATTTCCCTGTCATCCTGACCAGAG
Seq A exon
AGCTTCTGTGTGGAGGATCCCCAGAGCCCTGTGCCAACGGAGGCACCTGCCTGAGGCTATCTCGGGGACAAGGGATCTGCCA
Seq C2 exon
GTGTGCCCCTGGATTTCTGGGTGAGACTTGCCAGTTTCCTGACCCCTGCAGGGATACCCAACTCTGCAAGAATGGTGGCAGCTGCCAAGCCCTGCTCCCCACACCCCCAAGCTCCCGTAGTCCTACTTCTCCACTGACCCCTCACTTCTCCTGCACCTGCCCCTCTGGCTTCACCGGTGATCGATGCCAAACCCATCTGGAAGAGCTCTGTCCACCTTCTTTCTGTTCCAACGGGGGTCACTGCTATGTTCAGGCCTCAGGCCGCCCACAGTGCTCCTGCGAGCCTGGGTGGACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000015468_CASSETTE1
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.000 A=0.000 C2=0.020
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
NO

							
A:
PF0000822=EGF=PU(67.7=75.0)

							
C2:
PF0000822=EGF=PD(29.0=9.0),PF0000822=EGF=WD(100=46.0),PF079748=EGF_2=PU(87.9=29.0)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000015612





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000015612f460A, ENSMUSP00000133574
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr6:32223005-32223086 
HIGH PSI
HsaEX0043415
(NOTCH4)
Human
(hg19)
chr6:32190782-32190863 
HIGH PSI
HsaEX0043415
(NOTCH4)
Rat
(rn6)
chr20:4330762-4330843 
ALTERNATIVE
RnoEX0060488
(Notch4)
Cow
(bosTau6)
chr23:26974041-26974122 
HIGH PSI
BtaEX6098200
(NOTCH4)
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
GAAGAGGGAGAGGAGATCCGG

				
R: 
AGAGTTGGGTATCCCTGCAGG

				
Band lengths: 
171-253

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






Retrieved from "https://vastdb.crg.eu/wiki/Special:VastDB"