MmuEX0034423 @ mm9
Exon Skipping
Gene
ENSMUSG00000028779 | Pef1
Description
penta-EF hand domain containing 1 [Source:MGI Symbol;Acc:MGI:1915148]
Coordinates
chr4:129784607-129802462:+
Coord C1 exon
chr4:129784607-129784894
Coord A exon
chr4:129798412-129798685
Coord C2 exon
chr4:129802307-129802462
Length
274 bp
Sequences
Splice sites
3' ss Seq
TGTGTCTGTTCTTCCTGCAGAGT
3' ss Score
12.59
5' ss Seq
AGGGTAAGT
5' ss Score
10.45
Exon sequences
Seq C1 exon
ATAACTACAGCTGGCCAGGCCCGAGGTCCGCTGCAGGTCACGAAAAATAAAAACAAACGAGCCACTACGGTCAACAACACTTCCGGACACTCCTACCCAGCCCTCTGTAAGGTACCAGCTCACCGCCTGTGACTTCCGTTTGTTGACCGGAAGATTGACAGCCTGTCCGTCCAATCACAATCGGACCCGCCCCAAAATCCAACCAGGAGCGGCATTGGGAGCAGGAAACTCCGCCTACCACAGTTGTGTGACGTCATAACCGCCATGGCCAGCTATCCAAACGGACAG
Seq A exon
AGTTGCCCAGGAGCTGCAGGACAGGTGCCTGGAGTACCCCCGGGGGGCTATTATCCTGGTCCTCCCCATGGTGGGGGCCAGTATGGCAGTGGACTGCCCCCAGGTGGTGGCTATGGAGCTCCTGCCCCTGGAGGACCCTATGGATACCCCAGTGCTGGAGGAGTCCCCTCGGGAACTCCAAGTGGACCATATGGCGGTATACCTCCAGGGGGTCCCTATGGTCAGCTACCTCCAGGGGGTCCCTACGGTACCCAGCCTGGACATTATGGACAGG
Seq C2 exon
GTGGTGTCCCCCCGAATGTGGATCCTGAGGCCTACTCCTGGTTCCAGTCAGTGGATGCCGATCACAGTGGCTATATCTCCCTCAAGGAGCTGAAGCAGGCCCTAGTCAACTCCAACTGGTCCTCATTCAATGACGAGACATGCCTCATGATGATAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028779_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=0.989 C2=0.189
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF134991=EF-hand_7=PU(71.4=94.3)
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAACGAGCCACTACGGTCAAC
R:
GTGATCGGCATCCACTGACTG
Band lengths:
299-573
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: