RnoEX0064805 @ rn6
Exon Skipping
Gene
ENSRNOG00000013972 | Pef1
Description
penta-EF hand domain containing 1 [Source:RGD Symbol;Acc:1359536]
Coordinates
chr5:148320438-148341062:+
Coord C1 exon
chr5:148320438-148320645
Coord A exon
chr5:148337037-148337334
Coord C2 exon
chr5:148340907-148341062
Length
298 bp
Sequences
Splice sites
3' ss Seq
CCTGTCTGTTCTTCCTACAGAGT
3' ss Score
11.77
5' ss Seq
AGGGTAAGT
5' ss Score
10.45
Exon sequences
Seq C1 exon
ACCGGACCCTCCATCTCTCCTGTTCCCCTGTTAGGTACCCACCCACCGCCTGTGACTTCCGTTTGTTAACCGGTAGATTGACAGCCTGGATGTCCAATTACAAGCGGAACTGACCCAAAGCCCAACGAGAGATGTTGAGAACAGGAAGTTCAGCCCACCACAATTGTATGACGTCATAACCGCCATGGCCAGCTATCCAGACGGGCAG
Seq A exon
AGTTACCCAGGAGCTGCAGGACAGGTACCTGGACCACACCCCGGTGGCTACTATCCTGGACCTCCCCATGGTGGGGGCCAGTATGGCAGTGGATTCCCCCCTGGTGGTTACGGAGCTCCTGCCCCTGGAGGACCCTATGGCTACCCCAGTGCTGGAGGAACCCCCTCTGGAACTCCAGGCGGACCATATGGCGGTGGACCTCCAGGAGGCCCCTATGGTGGTGGACCTCCAGGAGGCCCCTATGGTCAGGCACATCCAAGTCCCTATGGTACCCAGCCGCCTGGACCTTATGGACAGG
Seq C2 exon
GTGGCGTCCCCCCCAATGTCGATCCTGAGGCCTACTCCTGGTTCCAGTCAGTGGATGCCGACCACAGTGGCTATATCTCACTCAAGGAGCTGAAGCAGGCCCTGGTCAACTCCAACTGGTCCTCATTCAATGATGAGACGTGCCTCATGATGATAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000013972-'0-5,'0-3,4-5
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=1.000 C2=0.245
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF134991=EF-hand_7=PU(71.4=94.3)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCATCTCTCCTGTTCCCCTGT
R:
CCTGCTTCAGCTCCTTGAGTG
Band lengths:
297-595
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]