MmuEX0035464 @ mm10
Exon Skipping
Gene
ENSMUSG00000026991 | Pkp4
Description
plakophilin 4 [Source:MGI Symbol;Acc:MGI:109281]
Coordinates
chr2:59214678-59266500:+
Coord C1 exon
chr2:59214678-59214814
Coord A exon
chr2:59214934-59215030
Coord C2 exon
chr2:59266388-59266500
Length
97 bp
Sequences
Splice sites
3' ss Seq
TCACGTTTTCTAATTATAAGGTG
3' ss Score
5.44
5' ss Seq
CAAGTAAGG
5' ss Score
7.66
Exon sequences
Seq C1 exon
GAGGAATGCCCGCCCCTGAACAGGGCTCACTGGTGGAGGAGGGGCAACCACAGACCCACCAGGAAGCTGTCTCCACTGGCCCAGGCATGGAACCCGAGACCACAGCCACCACTATTCTAGCTTCCGTGAAGGAGCAG
Seq A exon
GTGTTTACATCCCTGAGTACATTGGGATGACTGGCATGGAGAGCTGCTCCAGCTGCCAGTCAGAGCCGGAACATGCAGGATACTTGTGCGTCTGCAA
Seq C2 exon
GAGCTTCAGTTTCAACGACTCACCCGAGAACTGGAAGTGGAAAGGCAGATTGTTGCCAGTCAGCTAGAAAGATGTAGGCTTGGAGCAGAATCGCCAAGCATCGCCAGCACCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000026991_MULTIEX1-2/5=1-4
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
5' UTR
No structure available
Features
Disorder rate (Iupred):
C1=0.997 A=0.444 C2=0.921
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Inclusion Isoform:
ENSMUST00000037903fB3031
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
Associated events
Other assemblies
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACCAGGAAGCTGTCTCCACTG
R:
GCGATTCTGCTCCAAGCCTAC
Band lengths:
174-271
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with pro-oncogenic impact when depleted: HeLa Enriched at 14 days (FC=1.258, FDR=0.000), PC9 No Change at 14 days (FC=1.248, FDR=0.033), Late Xenograft No change (FC=1.363, FDR=0.273).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types