Special

MmuEX0036893 @ mm9

Exon Skipping

Gene
ENSMUSG00000015474 | Ppt2
Description
palmitoyl-protein thioesterase 2 [Source:MGI Symbol;Acc:MGI:1860075]
Coordinates
chr17:34763404-34764380:-
Coord C1 exon
chr17:34764270-34764380
Coord A exon
chr17:34763750-34763940
Coord C2 exon
chr17:34763404-34763557
Length
191 bp
Sequences
Splice sites
3' ss Seq
GGCCTCTCGTTCCTTCTCAGGTG
3' ss Score
9.49
5' ss Seq
GAGGTCTGT
5' ss Score
5.39
Exon sequences
Seq C1 exon
GTCCGTCATCCCACTGCGCGCTCTCCTTGTCCCCCTATCCCCGTATCCGGTGGATTACAGACTAGGTACAAGTAAACAGCTGGCGGAACGAAGCCTCCAGACACAGACCAG
Seq A exon
GTGGGAGCATGCCGGGGCTATGGAGGCAGAGGCTTCCTTCGGCTTGGGCTTTGCTTCTCCTGCCGTTCCTGCCGCTGCTGATGCCCGCAGCCCCCGCAGCCCACCGCGGGTCCTACAAGCCCGTGATCGTGGTGCACGGGCTCTTTGACAGTTCATACAGCTTCCGCCACCTGCTGGACTATATCAATGAG
Seq C2 exon
ACACACACCGGGACTGTGGTGACAGTGCTTGATCTCTTCGATGGCAGAGAGAGTTTGCGGCCCCTGTGGGAACAGGTACAAGGGTTCCGAGAGGCTGTGGTCCCCATCATGGAAAAGGCCCCTGAAGGAGTGCACCTCATCTGCTACTCCCAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000015474-'3-11,'3-9,8-11
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion (Ref, Alt. ATG (<=10 exons))





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=NA A=0.000 C2=0.000
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
NA

							
A:
PF0208910=Palm_thioest=PU(10.2=44.3)

							
C2:
PF0208910=Palm_thioest=FE(19.3=100)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000068071





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000125937, ENSMUSP00000127372, ENSMUSP00000127707, ENSMUSP00000127726, ENSMUSP00000127745, ENSMUSP00000129421, ENSMUSP00000131243, ENSMUSP00000132006, ENSMUSP00000132339
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr6:32154587-32154777 
ALTERNATIVE
HsaEX0049848
(PPT2)
Human
(hg19)
chr6:32122364-32122554 
HIGH PSI
HsaEX0049848
(PPT2)
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
chr23:27035253-27035452 
HIGH PSI
BtaEX6098223
(PPT2)
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
CTCTCCTTGTCCCCCTATCCC

				
R: 
CCTGGGAGTAGCAGATGAGGT

				
Band lengths: 
245-436

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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