MmuEX0039505 @ mm10
Exon Skipping
Gene
ENSMUSG00000020696 | Rffl
Description
ring finger and FYVE like domain containing protein [Source:MGI Symbol;Acc:MGI:1914588]
Coordinates
chr11:82812400-82871210:-
Coord C1 exon
chr11:82871008-82871210
Coord A exon
chr11:82818416-82818603
Coord C2 exon
chr11:82812400-82812810
Length
188 bp
Sequences
Splice sites
3' ss Seq
CTCTCCCTCTCCTGTGCCAGATT
3' ss Score
9.5
5' ss Seq
AAGGTAAGT
5' ss Score
11
Exon sequences
Seq C1 exon
GGGGCCCACGCCGAGCCGCGGGGGACTGGGTTAGTGGGGGTGGACTTCCTGGGGCCGCCCGTGAGGATGTGGTTCGGGCACCCGGGCGCCCCTCAGGCGTGGAGTTGCGGCGGCCGCAGACCCCGCAGCCCCGCTGCCTGAGTGGCCGCGGTCGGGATCTGGCCTCTGCCGTCCCAGGTGTGGCCCGGCAGCGTCCGCGGCAG
Seq A exon
ATTTTATCATGTGGGCATCCTGCTGCAACTGGTTCTGCTTGGATGGACAGCCTGAGGAGGCACCACCACCACAGGGAGCCAGGACACAAGCCTATTCCAACCCTGGGTACAGCTCCTTCCCTTCCCCCACAGGCTCGGAACCTAGCTGCAAGGCCTGCGGGGTCCATTTCGCAAGCACAACCCGGAAG
Seq C2 exon
CAGACCTGCTTGGACTGTAAGAAAAACTTCTGCATGACTTGTTCGAGCCAAGAGGGGAATGGGCCACGCCTCTGCCTTCTCTGCCTACGGTTCCGAGCCACGGCCTTCCAGCGGGAGGAGCTCATGAAGATGAAGGTGAAGGACCTGAGGGACTATCTCAGCCTCCACGACATCTCTACGGAAATGTGCCGGGAGAAAGAGGAGCTGGTGTTCCTGGTGCTTGGCCAGCAGCCTGTAATTTCTGAGGCGGACAGGACTCGTGTTCCCCACTTGCCCCAGGCCTTCCCTGAGCAGCAGGCCTTCCTGACCCAGCCTCAAACCAGCACAGTGCCTCCTACCTCACCTGGCCTCCCTTCTTCACCTGCACAAGTCACCTCTGTCCCCTTAGCCCAGGATCAGGAAACTCAGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000020696-'0-17,'0-14,16-17
Average complexity
C2
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=NA A=0.152 C2=0.359
Domain overlap (PFAM):
C1:
NA
A:
PF0231811=FYVE_2=PU(29.9=31.7)
C2:
PF0231811=FYVE_2=PD(67.2=32.8),PF102084=Armet=WD(100=48.2)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGACTGGGTTAGTGGGGGTG
R:
CATTCCCCTCTTGGCTCGAAC
Band lengths:
242-430
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types