MmuEX0053253 @ mm10
Exon Skipping
Gene
ENSMUSG00000020335 | Zfp354b
Description
zinc finger protein 354B [Source:MGI Symbol;Acc:MGI:1351476]
Coordinates
chr11:50928564-50930985:-
Coord C1 exon
chr11:50930901-50930985
Coord A exon
chr11:50929377-50929503
Coord C2 exon
chr11:50928564-50928659
Length
127 bp
Sequences
Splice sites
3' ss Seq
GGAACGGCTTTCCCTCACAGGTG
3' ss Score
8.06
5' ss Seq
TGGGTAAGA
5' ss Score
8.91
Exon sequences
Seq C1 exon
ATCGCATTTTTGGACACTGTACCCATCTTTGAGAAAAGCCCAGAAAGTGAGCATGGCTCCAGAGCAAAGGGAAGGGAAGTCTCAG
Seq A exon
GTGTCGGTGACGTTTGAAGACGTGGCCGTGCTCTTTACCCGGGATGAGTGGAAGAAGCTGGTTCCCTCTCAGAGAAGCCTGTACCGTGAGGTGATGCTGGAGAACTACAGCAACCTGGCCTCGCTGG
Seq C2 exon
GATTCCCATTTACCAAACCAAAGATGATCTCCGTGTTGCAACAAGGAGAAGAACCCTGGAAATCAGAGAAAGAAAGTCATGGATGCTCCCCTCTTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000020335-'4-6,'4-5,6-6=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.909 A=0.000 C2=0.455
Domain overlap (PFAM):
C1:
NO
A:
PF0135222=KRAB=WD(100=95.3)
C2:
PF0135222=KRAB=PD(0.1=0.0)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGCATTTTTGGACACTGTACCC
R:
AAGAGGGGAGCATCCATGACT
Band lengths:
178-305
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types