MmuEX6011769 @ mm10
Exon Skipping
Gene
ENSMUSG00000030539 | Sema4b
Description
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B [Source:MGI Symbol;Acc:MGI:107559]
Coordinates
chr7:80216948-80219336:+
Coord C1 exon
chr7:80216948-80217099
Coord A exon
chr7:80218883-80219064
Coord C2 exon
chr7:80219186-80219336
Length
182 bp
Sequences
Splice sites
3' ss Seq
TGTCCCCTCTTCTCCTACAGGGC
3' ss Score
13.32
5' ss Seq
GTGGTGAGA
5' ss Score
5.46
Exon sequences
Seq C1 exon
ACCCTGCCTTTGTGGCCTCGGCCTACGTCCCCGAGAGCCTGGGCAGCCCCATAGGTGATGATGATAAGATCTACTTCTTCTTCAGCGAGACGGGCCAGGAGTTTGAGTTCTTTGAGAACACCATCGTGTCCCGAGTTGCCCGAGTCTGTAAG
Seq A exon
GGCGATGAGGGTGGAGAGCGGGTGTTGCAGCAACGCTGGACCTCCTTTCTCAAGGCTCAGCTCCTGTGCTCCCGGCCTGATGATGGCTTTCCCTTTAACGTGCTACAAGATGTCTTCACCCTGAACCCCAACCCTCAGGATTGGCGCAAGACCCTTTTCTATGGGGTCTTTACCTCCCAGTG
Seq C2 exon
GCACAGAGGGACCACAGAAGGCTCTGCCATCTGCGTCTTCACCATGAATGATGTGCAGAAGGCCTTTGACGGCCTGTACAAGAAAGTAAACAGAGAGACACAGCAGTGGTATACCGAGACCCACCAGGTGCCCACACCGCGGCCGGGAGCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000030539-'18-18,'18-16,20-18
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.176
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(11.4=100)
A:
PF0140314=Sema=FE(13.7=100)
C2:
PF0140314=Sema=FE(11.4=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTACGTCCCCGAGAGCCTG
R:
GGGTCTCGGTATACCACTGCT
Band lengths:
252-434
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types