MmuEX7004183 @ mm10
Exon Skipping
Gene
ENSMUSG00000030660 | Pik3c2a
Description
phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide [Source:MGI Symbol;Acc:MGI:1203729]
Coordinates
chr7:116405860-116443449:-
Coord C1 exon
chr7:116443304-116443449
Coord A exon
chr7:116417453-116418581
Coord C2 exon
chr7:116405860-116405963
Length
1129 bp
Sequences
Splice sites
3' ss Seq
ATGTTTATTTTTCTGTGTAGGTC
3' ss Score
10.28
5' ss Seq
CAGGTATAG
5' ss Score
8.73
Exon sequences
Seq C1 exon
GTGCAGAGCTCGGCTGGCCGCGGAGTCAGTCGAAGCTCTCCTCAGCGGCCGGCTGAGCCAGCTGAGGCGGGAGAAAAACATGGCTCGGACCTTGGAGGGCGCGAAGGCTCGGGTTGCGGTGAAGACCAAGACTCCCGCAGCGTGAG
Seq A exon
GTCCTGGTATTTTGGAATCTACAAGAAAAAAAGATTAAGAGGTTTGTTCTCTTTTGTGGACATGGCTCAGATTTCCAACAACAGTGAATTTAAACAATGTTCATCTTCACATCCAGAACCAATAAGAACCAAAGATGTGAACAAAGCAGAAGCGTTACAGATGGAAGCAGAAGCCTTAGCAAAACTGCAGAAGGATAGACAAATGACTGACAGCCCAAGAGGCTTTGAGCTGTCTAGCAGCACTAGACAAAGAACACAAGGTTTTAACAAACAGGATTATGATCTCATGGTGTTTCCTGAGTTGGATTCCCAAAAAAGAGCAGTAGATATTGATGTAGAAAAGCTCACCCAGGCTGAACTTGAGAAGATATTGCTGGACGACAATTTTGAAACTAGAAAACCTCCTGCATTGCCAGTTACTCCTGTTCTGAGCCCTTCGTTCTCAACACAGCTGTATCTTAGACCTAGTGGTCAAAGAGGCCAGTGGCCCCCTGGATTATGCGGGCCTTCCACGTACACTTTACCTTCTACTTATCCTTCAGCATACAGTAAACAGGCCACATTCCAGAATGGCTTCAGTCCAAGGATGCCCACTTTTCCATCAACAGAGTCTGTATATTTAAGACTTCCTGGACAGTCTCCATATTTTTCATATCCTTTGACACCTGCCACACCATTTCATCCACAAGGAAGTTTACCAGTCTATCGGCCACTAGTCAGTCCTGACATGGCAAAACTATTTGAAAAAATAGCAAGTACCTCAGAATTTTTAAAAAATGGGAAAGCAAGGACTGATTTGGAGATAGCAAACTCGAAAGCTTCAGTCTGCAATCTACAGATATCTCCAAAGTCTGAAGACATCAATAAGTTTGATTGGTTAGACTTGGATCCTCTGAGTAAGCCTAAGGTAGACTATGTGGAGGTGTTAGAACATGAAGAAGAGAAGAAGGATCCAGTTTTGCTAGCAGAGGATCCTTGGGATGCTGTTCTTCTTGAAGAGAGATCGCCAAGTTGTCACCTAGAAAGAAAGGTGAATGGAAAATCCCTTTCTGGGGCAACTGTAACAAGAAGCCAGTCTTTAATCATTCGGACAGCTCAATTTACAAAAGCCCAGGGCCAAGTATCTCAG
Seq C2 exon
AAAGACCCAAATGGGACCAGTAGTTTGCCAACTGGAAGTTCTCTTCTACAAGAATTTGAAGTACAGAATGACGAGGTGGCAGCTTTTTGTCAATCCATTATGAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000030660-'4-11,'4-8,9-11=AN
Average complexity
A_C2
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence exclusion (Ref, Alt. ATG (<=10 exons))
No structure available
Features
Disorder rate (Iupred):
C1=NA A=0.287 C2=0.309
Domain overlap (PFAM):
C1:
NA
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGGCCGCGGAGTCAGTC
R:
TGGATTGACAAAAAGCTGCCAC
Band lengths:
230-1359
Functional annotations
There are 1 annotated functions for this event
PMID: 12719431
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, de novo protein sequencing by mass spectrometry, electrophoretic mobility supershift assay, inhibitor, protein kinase assay, western blot. ELM ID: ELMI003240; ELM sequence: LQVSPK; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types