MmuEX7006266 @ mm10
Exon Skipping
Gene
ENSMUSG00000032303 | Chrna3
Description
cholinergic receptor, nicotinic, alpha polypeptide 3 [Source:MGI Symbol;Acc:MGI:87887]
Coordinates
chr9:55010111-55022288:-
Coord C1 exon
chr9:55022179-55022288
Coord A exon
chr9:55015137-55016148
Coord C2 exon
chr9:55010111-55012929
Length
1012 bp
Sequences
Splice sites
3' ss Seq
GCCCCACTGTGTCTTCCCAGCGC
3' ss Score
8.87
5' ss Seq
GAGGTAAAT
5' ss Score
8.39
Exon sequences
Seq C1 exon
ATCTGGAATGACTACAAGCTGAAATGGAAACCCTCTGACTACCAAGGGGTGGAGTTCATGCGAGTCCCTGCAGAGAAGATCTGGAAACCAGACATCGTGCTTTACAACAA
Seq A exon
CGCCGATGGGGATTTCCAAGTGGATGACAAAACCAAAGCTCTACTCAAGTACACAGGAGAAGTGACTTGGATCCCTCCGGCCATCTTTAAGAGCTCATGCAAAATCGATGTGACCTACTTCCCGTTTGACTACCAAAACTGCACCATGAAGTTCGGCTCCTGGTCCTACGACAAGGCAAAGATCGACCTGGTCCTCATTGGCTCCTCCATGAACCTCAAGGACTATTGGGAAAGTGGCGAGTGGGCCATCATTAAAGCCCCGGGCTACAAACATGAAATCAAGTACAACTGCTGTGAGGAGATCTACCAAGACATCACGTACTCGCTATACATTCGCCGCCTGCCGCTGTTCTACACCATCAACCTCATCATTCCGTGCCTGCTCATCTCCTTCCTCACTGTGCTCGTCTTCTACCTGCCCTCCGACTGTGGGGAGAAGGTGACGCTCTGCATCTCCGTGCTCCTCTCCCTGACGGTCTTTCTCCTCGTGATCACCGAGACCATCCCTTCCACCTCACTGGTCATCCCCTTGATCGGGGAGTACCTCCTCTTCACTATGATTTTTGTCACCTTGTCCATCGTCATCACAGTCTTTGTGCTCAACGTGCACTACAGAACTCCGACCACACACACGATGCCCACTTGGGTCAAGGCTGTGTTTTTGAACCTTCTCCCCAGGGTCATGTTTATGACTAGGCCAACCAGCACCGAGGAAGACGCCCCAAAGACGAGGAACTTCTACGGTGCTGAGCTCTCAAACCTGAACTGCTTCAGCCGTGCAGACTCCAAAAGCTGCAAGGAAGGCTACCCCTGCCAAGATGGGACATGTGGCTATTGCCACCACCGTAGGGTAAAAATCTCAAATTTCAGTGCCAACCTCACAAGAAGCTCCAGTTCTGAGTCTGTTGATGCTGTGTTGTCCCTGTCTGCTCTGTCACCAGAAATCAAAGAAGCCATCCAAAGTGTGAAGTATATTGCTGAAAATATGAAAGCACAGAATGTAGCCAAAGAG
Seq C2 exon
ATTCAAGATGATTGGAAGTATGTTGCCATGGTGATTGATCGCATTTTTCTCTGGGTTTTCATCCTGGTGTGCATTTTAGGAACTGCAGGATTATTTCTGCAACCCTTGATGGCCAGAGATGACACATAAACTGTGACCTGTTCGCCAAGGCTCGCTCACCTGTGCTGATGCAAGGGCACTTTCTCAATAGCCCCACACTTTATCCGATCACTGCTTGGAGTTTGCTATTTCAAGGGTTATTTTCCACTCATCTTGATCTTTTTTTGTTGTTGTTGTTAAAAAGAAATGTGGGAGAAAAACATCTAGTTATTTACTAAGGATAAATGAACATTTCACTATCCCCATGATTGAAAACTAGATAGAAATTTTTTCTGTTCATAAATATTAAATCCATTAGCTCAGAATCTCTATAGAACTCATTTGTTCAGCCCATAAACTACTTGAAGTTATAAAGTATTCCAGCAGCACCCCATCACTTCCATTCTTTACTATTTTTTCAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000032303-'5-5,'5-4,6-5=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.006 C2=0.000
Domain overlap (PFAM):
C1:
PF0293118=Neur_chan_LBD=FE(17.4=100)
A:
PF0293118=Neur_chan_LBD=PD(55.6=34.0),PF0293211=Neur_chan_memb=PU(86.7=63.6)
C2:
PF0293211=Neur_chan_memb=PD(12.5=72.1)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGAATGACTACAAGCTGAAATGGA
R:
CCCTTGAAATAGCAAACTCCAAGC
Band lengths:
343-1355
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types