RnoEX0021200 @ rn6
Exon Skipping
Gene
ENSRNOG00000013829 | Chrna3
Description
cholinergic receptor nicotinic alpha 3 subunit [Source:RGD Symbol;Acc:2345]
Coordinates
chr8:59592403-59602961:-
Coord C1 exon
chr8:59602852-59602961
Coord A exon
chr8:59596364-59597375
Coord C2 exon
chr8:59592403-59594135
Length
1012 bp
Sequences
Splice sites
3' ss Seq
GACCCACTGTGTCTTCACAGCGC
3' ss Score
9.78
5' ss Seq
GAGGTAAAT
5' ss Score
8.39
Exon sequences
Seq C1 exon
ATCTGGAATGACTACAAGCTGAAATGGAAACCCTCTGACTACCAAGGGGTGGAGTTCATGCGTGTTCCTGCAGAGAAGATCTGGAAACCAGACATCGTACTGTACAACAA
Seq A exon
CGCTGATGGGGATTTCCAGGTGGATGACAAGACCAAAGCTCTACTCAAGTACACAGGAGAAGTGACTTGGATCCCGCCGGCCATCTTTAAGAGCTCATGCAAAATCGACGTGACCTACTTCCCATTCGACTACCAAAACTGCACCATGAAGTTCGGCTCCTGGTCCTACGACAAGGCAAAGATCGACCTGGTCCTCATCGGCTCCTCCATGAACCTCAAGGACTACTGGGAGAGTGGCGAGTGGGCTATCATTAAAGCCCCGGGCTACAAACATGAAATCAAGTACAACTGCTGTGAGGAGATCTACCAAGACATCACGTACTCGCTGTACATCCGTCGCCTGCCGCTGTTCTACACCATCAACCTCATCATCCCCTGCCTGCTCATCTCCTTCCTCACTGTGCTTGTCTTCTACCTGCCCTCCGACTGTGGGGAGAAGGTGACACTCTGCATCTCTGTGCTCCTCTCCCTGACTGTCTTTCTCCTGGTGATCACCGAGACCATTCCTTCCACCTCGCTGGTCATCCCCCTGATTGGGGAGTACCTCCTCTTCACTATGATTTTTGTCACCTTGTCCATTGTCATCACAGTCTTTGTGCTCAATGTGCACTATAGAACTCCAACCACACACACCATGCCCACTTGGGTCAAGGCCGTGTTCTTGAACCTGCTCCCCAGGGTCATGTTTATGACTAGGCCGACCAGTGGTGAGGGGGACACTCCTAAGACGAGGACCTTCTACGGCGCTGAGCTCTCAAACCTGAACTGCTTCAGCCGTGCAGACTCCAAAAGCTGCAAGGAAGGCTACCCCTGCCAAGATGGGACCTGTGGCTACTGCCACCACCGTAGGGTAAAAATCTCAAATTTCAGTGCCAACCTCACAAGAAGCTCCAGTTCTGAGTCTGTCGACGCTGTGTTGTCCCTCTCTGCCCTGTCACCAGAAATCAAAGAAGCCATCCAAAGTGTGAAGTACATTGCCGAAAACATGAAAGCACAGAATGTAGCCAAAGAG
Seq C2 exon
ATTCAAGATGATTGGAAGTACGTTGCCATGGTGATTGATCGCATCTTTCTCTGGGTTTTCATCCTGGTGTGCATTTTAGGAACGGCGGGATTATTTCTGCAACCCTTGATGGCCAGAGATGACACATAGACTGTGCACTGTTCATCGAGGCTCACCTGTGCTGACGCAAGGGCACTTCCTCAATAGCACTATACTTTATCTGATCACTGGCTTGGAGTTTGCTATTTCAAGGGTTATTTTCAACTCATCTTGATTTTTCGTAAAAAAGAAATGTGGGAGACAAATGTCTAGTTATTTATCATGGGTAAATGAACATTTCACTACCCCTGTGATTGAAACTTTAGGTAGAAAAATTTTCTGTTCATAAATATTAACTCCATTAGTGCAGGATCTCCATAGAACTCATTTGCTCAGCCCATAAACTTCTTGAAATCGTAGTGTATTCAGCAGCGCCTGTTACTTCCGTTCTTTACTATCTTTCCATGTTGGCATTTGTGTTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000013829_MULTIEX1-2/2=1-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.006 C2=0.000
Domain overlap (PFAM):
C1:
PF0293118=Neur_chan_LBD=FE(17.4=100)
A:
PF0293118=Neur_chan_LBD=PD(55.6=34.0),PF0293211=Neur_chan_memb=PU(86.7=63.6)
C2:
PF0293211=Neur_chan_memb=PD(12.5=72.1)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Mouse
(mm9)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGGAGTTCATGCGTGTTCCT
R:
GACATTTGTCTCCCACATTTCTT
Band lengths:
350-1362
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]