MmuINT0042037 @ mm10
Intron Retention
Gene
ENSMUSG00000035403 | Crb2
Description
crumbs family member 2 [Source:MGI Symbol;Acc:MGI:2679260]
Coordinates
chr2:37793099-37794646:+
Coord C1 exon
chr2:37793099-37793867
Coord A exon
chr2:37793868-37794529
Coord C2 exon
chr2:37794530-37794646
Length
662 bp
Sequences
Splice sites
5' ss Seq
CAGGTGCGG
5' ss Score
9.13
3' ss Seq
GGACTTGCTTTTCTCTGCAGGTT
3' ss Score
10.09
Exon sequences
Seq C1 exon
GTGTGGCCGAGGCCACGTTCCGCGAGGGCCCTCCTGCTGTGTTCACAGGCCACAACGTGTCCTCATCGCTCAGCGGGCTCACCCTGGCCTTCCGCACGCGCGACTCCGAGGCTGGGCTACTGCGCGCCGTCTCCGCCGCAGGTGCCCACTCCAATATCTGGTTGGCGGTGCGCAACGGCTCGCTGGCAGGAGATGTGGCGGGTTCGGTGCTGCCCGCGCCCGGGCCGCGCGTGGCCGACGGCGCCTGGCATCGCGTGCGCCTAGCCCGGGAGTTCCCACAGGCCGCTGCCTCGCGCTGGCTGCTGTGGCTGGACGGCGCGGCGACACCCGTGGCCTTGCACGGCTTGGGCGGCGACCTGGGCTTTCTGCAGGGTCCGGGTGCAGTGCCTCTGCTACTGGCTGAGAACTTCACGGGCTGCCTGGGCCGCGTGGCACTTGGCGACTTCCCGTTACCCTTGGCGCCACCGCGGTCTGGCACGGTGTCTGGGGCGCGCGAGCACTTCGTGGCCTGGCCCGGGTCTCCGGCCGTGAGTCTCGGCTGCCGTGGCGGACCCGTATGTTCACCCTCGCCCTGCCTGCACGGTGGCGCCTGCCGCGACCTCTTCGACGCCTTTGCTTGCTCCTGCGGCCCCGCCTGGGAAGGACCCCGCTGCGAGATCCGCGCTGACCCATGTCGCTCCACTCCCTGTGTCCGGGGCCAATGCCATGCGCGCCCCGACGGCCGCTTCGAGTGTCGCTGCCCTCCGGGCTTCTCCGGCCCGCGCTGCAG
Seq A exon
GTGCGGGTGACAAGGGGACCTGACCTGGGCATAGCAGTGGGTAGAGATGACTGCTGATTTACCGTGGGTGGAGTTTGGAGGGAAAAAGTTCTTCATAGCTTGTTCAGTTTGGTGTCCATAAAGATAAAATACAAGCCGGGCGTGGTGGTGCACGCCTTTAATCCCAGCACCTGGGAGGCAGAGGCAGGCGGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAAAGTGGGTTCCAGGACAGCCAGGGCTATACAGAGAAACCCTGTCTTAAAAAAAAAAAAAAAAAAAAAAGACAAAATACAGGTTATAATAGTTGGCAAAATTTGAATTTCAGGACGATGTTGTGGCTTGGGTAGAATTAGGAGGGCAGGACGAATCAGGGAGCATGGTTTGCAGCAAGCTGCCTTTGAAACTCCCGTTTCCTTCCGGTCCAGAGCTGTCTAGCTCTCCTCTCTCCTCATGTTTAGCAACTGTTTTGCGCTGCCCTTTAAAGTCAGCACGAATCAAAGGCCTCAGCTCTTGTGGAGTTGGGGGAAGCGGCAGAATGACAGCCCTGCGTTCCTTTGCTTCCTGGGGAAGGCTGTTGAAGGCAGGCCTGCATTCCCCAGAGAGCACCACTATGGCCAGCTCCCAGCACCTCACTGGACTTGCTTTTCTCTGCAG
Seq C2 exon
GTTGCCTGTCCTGCCTCAGGGATGCAACCTCAACTCCACATGCAAGGATGGCGCCCCTTGTGAAGGCGGCCCCTTAGGCACCAACTGCAGTTGCCAGGAAGGTCTTGCTGGCCTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000035403:ENSMUST00000050372:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.008 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0221019=Laminin_G_2=WD(100=46.3),PF0000822=EGF=WD(100=12.1),PF126612=hEGF=WD(100=5.1)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CACTTGGCGACTTCCCGTTAC
R:
CTGAGGCAGGACAGGCAAC
Band lengths:
356-1018
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types