Special

MmuINT0055821 @ mm10

Intron Retention

Gene
Description
EGF-like domain 8 [Source:MGI Symbol;Acc:MGI:1932094]
Coordinates
chr17:34614669-34615001:-
Coord C1 exon
chr17:34614879-34615001
Coord A exon
chr17:34614779-34614878
Coord C2 exon
chr17:34614669-34614778
Length
100 bp
Sequences
Splice sites
5' ss Seq
CAGGTGAGA
5' ss Score
9.22
3' ss Seq
AGCCTGCTTCTGTGTTCTAGGAC
3' ss Score
10.23
Exon sequences
Seq C1 exon
TTTGGGAGTGTGCTCCAAGCAGACGCTGCTGGTTCCTCTCCGTTACAACGAGTCCTATAGTCAACCGGTGTACAAACCCTACCTGACCTTGTGTGCGGGGAGGCGCATATGTAGCACCTACAG
Seq A exon
GTGAGAGGTGAAGCCCCTAAGGCTCCCAGGTCTCAAAGGGACCCCAAGAGGCAAGTCCAGGATGGGTACCTACAGATATGAGCCTGCTTCTGTGTTCTAG
Seq C2 exon
GACCACATACCGTGTGGCCTGGCGGGAGGTGAGGCGGGAGGTACCACAGACACACGTGGTGTGCTGTCAGGGCTGGAAGAAGCCACACCCAGGAGCTCTCACCTGTGATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000015467:ENSMUST00000015611:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact

ORF disruption upon sequence inclusion

No structure available
Features
Disorder rate (Iupred):
  C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):

C1:
PF075468=EMI=PU(57.7=97.6)
A:
NA
C2:
PF075468=EMI=PD(40.8=76.3)


Main Inclusion Isoform:
NA


Main Skipping Isoform:


Other Inclusion Isoforms:
NA


Other Skipping Isoforms:
Associated events
Other assemblies
Conservation
Chicken
(galGal4)
ALTERNATIVE
Chicken
(galGal3)
ALTERNATIVE
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGACGCTGCTGGTTCCTCTC
R:
CATCACAGGTGAGAGCTCCT
Band lengths:
213-313
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Pre-implantation embryo development
  • Ribosome-engaged transcriptomes of neuronal types
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types