MmuINT0067132 @ mm10
Intron Retention
Gene
ENSMUSG00000026787 | Gad2
Description
glutamic acid decarboxylase 2 [Source:MGI Symbol;Acc:MGI:95634]
Coordinates
chr2:22684959-22685469:+
Coord C1 exon
chr2:22684959-22685066
Coord A exon
chr2:22685067-22685379
Coord C2 exon
chr2:22685380-22685469
Length
313 bp
Sequences
Splice sites
5' ss Seq
AAGGTACGT
5' ss Score
10.75
3' ss Seq
TCCTATTTTCTTCCTTTTAGCCT
3' ss Score
9.91
Exon sequences
Seq C1 exon
GGGACTACTGGGTTTGAGGCACACATTGATAAGTGTTTGGAGCTAGCAGAGTATTTATACACTATCATAAAAAACCGAGAAGGCTATGAAATGGTGTTTGATGGGAAG
Seq A exon
GTACGTGTTCAGCATTTCAACAATCACCATGAACATCCTCGGTTCCAGTGCTTACCCCTGGTGTTTTCTGGTGTCAGTAATGAGCTTTCTGGCTAAGTGGGACCATTACAGGGGCAAGTGATAGATGGTGCGGTGGTTCCTTTGAGCTGGGATAAAGAGTGGACTCACAACTCTGAAGAATGTGAAAGGGAAATATGCCAAAGGAAATCACTCATTTAAATACAGGAGTTGTCTTCTGAACCTGAAGAATCAAAACATTTGAACCCTCCTTGGAATTTTAGACCAGGCCTGAATCCTATTTTCTTCCTTTTAG
Seq C2 exon
CCTCAACACACAAATGTCTGCTTCTGGTTTGTACCTCCTAGTTTGCGCACTCTGGAAGACAATGAAGAGAGAATGAGCCGCCTCTCAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000026787:ENSMUST00000028123:14
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0028214=Pyridoxal_deC=FE(9.4=100)
A:
NA
C2:
PF0028214=Pyridoxal_deC=PD(2.7=33.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGGACTACTGGGTTTGAGGCA
R:
CTTTGAGAGGCGGCTCATTCT
Band lengths:
198-511
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types