MmuINT0097674 @ mm10
Intron Retention
Gene
ENSMUSG00000057751 | Megf6
Description
multiple EGF-like-domains 6 [Source:MGI Symbol;Acc:MGI:1919351]
Coordinates
chr4:154267190-154267561:+
Coord C1 exon
chr4:154267190-154267318
Coord A exon
chr4:154267319-154267432
Coord C2 exon
chr4:154267433-154267561
Length
114 bp
Sequences
Splice sites
5' ss Seq
AGGGTAAGC
5' ss Score
9.04
3' ss Seq
TCATTCTACACTTCCCACAGCCT
3' ss Score
7.28
Exon sequences
Seq C1 exon
CCTGCGTCAGTGGCATGTTTGGGGTTCACTGTGAGGAGCACTGTGCCTGCCGGAAAGGTGCCACCTGTCACCATGTCACGGGGGCCTGCCTCTGTCCCCCAGGATGGAGGGGCTCACACTGTGAACAGG
Seq A exon
GTAAGCAGAGATAAGTTTATGGACCATTGGGAGGGAGCTGGTGCTGCACAGCCATCTCGGGGATCCCCACTGCTCCGTTCCCAGAAAGCTTACCTCATTCTACACTTCCCACAG
Seq C2 exon
CCTGTCCACGTGGCTGGTTTGGAGAGGCCTGTGCTCAGCGTTGCCACTGCCCGCCTGGTGCCTCCTGCCACCATGTCTCTGGAGAGTGCCACTGTCCACCCGGCTTCACTGGGCCTGGCTGTGAGCAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000057751:ENSMUST00000030897:27
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0005319=Laminin_EGF=PU(63.0=65.9)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCAGTGGCATGTTTGGGGTTC
R:
ACAGCCAGGCCCAGTGAA
Band lengths:
245-359
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types