MmuINT0103445 @ mm9
Intron Retention
Gene
ENSMUSG00000033196 | Myh2
Description
myosin, heavy polypeptide 2, skeletal muscle, adult [Source:MGI Symbol;Acc:MGI:1339710]
Coordinates
chr11:66986927-66988601:+
Coord C1 exon
chr11:66986927-66987070
Coord A exon
chr11:66987071-66988444
Coord C2 exon
chr11:66988445-66988601
Length
1374 bp
Sequences
Splice sites
5' ss Seq
TACGTGAGT
5' ss Score
7.77
3' ss Seq
TTCTCTCTTTCTTTATACAGACC
3' ss Score
9.96
Exon sequences
Seq C1 exon
ACCCTGACCGTGAAAGAAGACCAGATCTTCCCCATGAACCCTCCCAAGTACGACAAGATCGAGGACATGGCCATGATGACCCACCTGCACGAGCCCGCTGTGCTGTACAACCTCAAAGAGCGTTATGCAGCCTGGATGATCTAC
Seq A exon
GTGAGTGACCCACACCAGGGGTCTTTAGTGTCATTACCATGTGCCCTAAAAGCCGAGGCACAAATGTGTCCTAAGTATCTCTCAGTCACTAGAAGTCACCAGCAGAATTACTAGTTATTAAAAAGTTCATTATTGAAACTGTAAGGCACATATTGTCTTCTCTCCCAAGGGCAGGCCATTGTCCGTCTGGCCATAGGCTTTATCACAGATCTGTAGATTAACCATGCTAAGGACGTCCCTTTCTTTCCCTTCCTATCTCTTTCCTCCCCCTCCATTCTTATCTCTTCCCCTTCCCTCCCTTCCCCTCTCTGTCAATTGCTGTTGCAGAATTTTGTCAATGAAGAAAAGATAAACACTCTGAAACATTTAAGAATGGAATTTTTAGGGGTTAAATTTCATTTTAGGGATTAAATCTCCCCACACAATAATACCAGGGTCTCTGTGTTTTGGTTCCTTTCATCTGTGTGATCTTTCCAGCATCCCCAGGCAGCCTTGAGTATTCTGCTCCGCTCTTCCCATCCCTGCATTCACACTATGTGTTTCCTGGTTATTCATTAACTTCCCCATCTACTGTGCTGACTGGGAATTTCTCAAAGGACTCTATGCTATCTATGTTTTAGTCTGACATCCTTGCAGGGTCCTAGAATATAATATGCATCCAATAACCATCTGAATGTAATGCATCTTATATTCATGTTTTTATACAGTTTAGCTAGAATTGATATTCAGCTGGGCAATACCCACACAGGTATACCAGATGTTTATGCTCTATAACCACTTTAATTGGCTAGTGACAGTCCTTAACCAGTTGACAGACAACTTTTAAATTCCTGTGTTTAATCCTTATGTATTTATTTGTTCTTTGTCGCTGTATAACTAAAACATACTTTACAGCATTTCCCAATACTGCCATATCCACACACTGTCCCACTCACTTTTGAAAGGCTTTTATCATGTTGTCTTAAAATATTCCAAAGTCCCCATCCTCTCTTACCGTTGAATACATTTATGTTCAAATTTATGTTCTAATAAACTCGAAGTGTCTCAGTTCTGCAAAGAAAATATACTCTTGTTTCTTTCAAACAACAAACAGCAAAAATCTCTCATAGTCCACATTTTAAAACAAATAGACAGACATGGTGTTGGTGCTCTTGCTGTGGGAAAACCATGACAAGTCCCAAGGGAAGCAGTAGGAACTCTGAACTGGTCATTGCCCTCAACTGATGCGGTTGCCTGTCAACCAGTCAGTTTCCATGCTGAAGACCGGGAGAAATGTACTCACAAGGGTCCTTCTTCTTCTTCTTAAATTCCAACCAAGTGAGTTGCACAGACAAGAAGGACCTTTGGATAGTCATTCTCTCTTTCTTTATACAG
Seq C2 exon
ACCTACTCAGGCCTCTTCTGTGTCACCGTCAACCCTTACAAATGGCTGCCGGTGTACAACCCCGAGGTGGTGGCGGCCTATCGAGGCAAAAAGCGCCAGGAGGCCCCGCCCCACATCTTCTCCATCTCTGATAACGCCTACCAGTTCATGCTAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000033196-Myh2:NM_001039545:4
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0273614=Myosin_N=PD(16.7=14.6),PF0006316=Myosin_head=PU(4.1=58.3)
A:
NA
C2:
PF0006316=Myosin_head=FE(7.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal4)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCCTGACCGTGAAAGAAGACC
R:
GTTAGCATGAACTGGTAGGCGT
Band lengths:
298-1672
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: