MmuINT0103453 @ mm9
Intron Retention
Gene
ENSMUSG00000020908 | Myh3
Description
myosin, heavy polypeptide 3, skeletal muscle, embryonic [Source:MGI Symbol;Acc:MGI:1339709]
Coordinates
chr11:66899847-66900168:+
Coord C1 exon
chr11:66899847-66899950
Coord A exon
chr11:66899951-66900029
Coord C2 exon
chr11:66900030-66900168
Length
79 bp
Sequences
Splice sites
5' ss Seq
GATGTAAGA
5' ss Score
5.46
3' ss Seq
AGCTGTTCTTTCCATTGCAGAGT
3' ss Score
9.87
Exon sequences
Seq C1 exon
AGCTGCTGCTGATTACGACCAACCCTTATGACTACCCGTTCATCAGCCAGGGCGAAATCCTGGTGGCCAGCATCGACGATGCGGAGGAGCTGTTAGCTACGGAT
Seq A exon
GTAAGAAGGACCACAGGAGGGCGGGAGAGCCTCCACAGAGGGAGGGCCACGGGCAGACAAGCTGTTCTTTCCATTGCAG
Seq C2 exon
AGTGCCATCGACATCCTGGGCTTCACCCCGGAGGAGAAATCTGGACTTTATAAACTGACAGGGGCTGTGATGCATTATGGGAACATGAAGTTCAAGCAGAAGCAGCGGGAGGAGCAGGCCGAGCCAGATGGCACTGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000020908-Myh3:NM_001099635:11
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.277
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(5.0=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(6.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGCTGCTGATTACGACCAACC
R:
CCCATAATGCATCACAGCCCC
Band lengths:
182-261
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: