MmuINT0103468 @ mm9
Intron Retention
Gene
ENSMUSG00000020908 | Myh3
Description
myosin, heavy polypeptide 3, skeletal muscle, embryonic [Source:MGI Symbol;Acc:MGI:1339709]
Coordinates
chr11:66906943-66907259:+
Coord C1 exon
chr11:66906943-66907088
Coord A exon
chr11:66907089-66907168
Coord C2 exon
chr11:66907169-66907259
Length
80 bp
Sequences
Splice sites
5' ss Seq
GAAGTACGT
5' ss Score
7.46
3' ss Seq
TTCTCTGTGTCCTGCCACAGGAA
3' ss Score
10.08
Exon sequences
Seq C1 exon
CTGGAGAGCTCCCTCGAACAAGAAAAGAAGCTGCGTGTGGACCTAGAAAGGAACAAAAGGAAGCTGGAGGGAGACCTGAAGCTTGCCCAAGAGTCCATATTAGATCTGGAGAACGACAAGCAACAGCTAGACGAGAGGCTCAAGAA
Seq A exon
GTACGTCCCCCCAAACACAGCTTCCATTTTGTGGGCGGCTGGGGATCCCATCTTCACGCGTTCTCTGTGTCCTGCCACAG
Seq C2 exon
GAAGGACTTTGAATATAGTCAGCTGCAAAGCAAAGTGGAAGATGAGCAGACCTTGAGCCTCCAGCTTCAGAAGAAAATCAAGGAGCTACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000020908-Myh3:NM_001099635:25
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.041 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=PD(0.1=0.0),PF0157614=Myosin_tail_1=PD(25.4=69.4),PF0003816=Filament=FE(17.6=100),PF0157614=Myosin_tail_1=PU(1.6=28.6)
A:
NA
C2:
PF0003816=Filament=FE(11.0=100),PF0157614=Myosin_tail_1=FE(3.5=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTGCGTGTGGACCTAGAAAG
R:
AGGCTCAAGGTCTGCTCATCT
Band lengths:
177-257
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: