MmuINT0103506 @ mm9
Intron Retention
Gene
ENSMUSG00000057003 | Myh4
Description
myosin, heavy polypeptide 4, skeletal muscle [Source:MGI Symbol;Acc:MGI:1339713]
Coordinates
chr11:67065684-67066202:+
Coord C1 exon
chr11:67065684-67065860
Coord A exon
chr11:67065861-67066056
Coord C2 exon
chr11:67066057-67066202
Length
196 bp
Sequences
Splice sites
5' ss Seq
GACGTGAGT
5' ss Score
9.22
3' ss Seq
CAAAGTCCCCTTTGATTTAGCTT
3' ss Score
4.28
Exon sequences
Seq C1 exon
GTGAAAAACCTGACAGAAGAGATGGCAGGCCTGGATGAAAATATCGCCAAGCTGACCAAGGAGAAGAAGGCCCTCCAGGAGGCCCACCAGCAGACCCTGGATGACCTGCAGGCAGAGGAGGACAAAGTCAACACCCTGACCAAAGCCAAAACCAAGCTGGAACAGCAAGTGGATGAC
Seq A exon
GTGAGTATGAAAAGCAGTCTTTTTCCTTATAAGGGATGACATACGCCATCATGGAACGAAGCCACCAGTGGAGCCCATGAGCCTCGAAATGCAGGCTGAGAGTCCTCGTGCTATTTGTTGTCACTTCTGAGTTTTTTCTTGAGTATCTAGCAAAGGACATAGGGTTCAGGGTTTAACAAAGTCCCCTTTGATTTAG
Seq C2 exon
CTTGAAGGATCACTGGAACAGGAAAAGAAGCTGAGGATGGACTTAGAAAGAGCCAAGAGGAAACTGGAGGGGGACCTGAAACTGGCCCAAGAGTCCACAATGGACATAGAAAACGATAAACAGCAGCTCGATGAGAAACTCAAAAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000057003-Myh4:NM_010855:23
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.983 A=NA C2=0.918
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=PD(28.5=93.2),PF0157614=Myosin_tail_1=FE(43.3=100),PF087025=Fib_alpha=PU(15.5=30.5)
A:
NA
C2:
PF0157614=Myosin_tail_1=PD(25.4=69.4),PF087025=Fib_alpha=FE(41.4=100),PF0157614=Myosin_tail_1=PU(1.6=28.6)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GATGGCAGGCCTGGATGAAAA
R:
CTCTTGGGCCAGTTTCAGGTC
Band lengths:
250-446
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: