MmuINT0103515 @ mm10
Intron Retention
Gene
ENSMUSG00000057003 | Myh4
Description
myosin, heavy polypeptide 4, skeletal muscle [Source:MGI Symbol;Acc:MGI:1339713]
Coordinates
chr11:67255568-67255956:+
Coord C1 exon
chr11:67255568-67255733
Coord A exon
chr11:67255734-67255831
Coord C2 exon
chr11:67255832-67255956
Length
98 bp
Sequences
Splice sites
5' ss Seq
AGCGTAAGC
5' ss Score
6.06
3' ss Seq
TTTCATCTGTTCTTCCGCAGAGG
3' ss Score
11.63
Exon sequences
Seq C1 exon
GTCCTGGCAGAATGGAAACAGAAGTACGAGGAAACTCAGGCTGAGCTTGAAGCCTCCCAGAAGGAGTCCCGCTCTCTGAGCACTGAGCTGTTCAAAGTGAAGAATGCCTATGAGGAGTCTCTGGATCAACTTGAGACGTTAAAGAGAGAGAACAAGAACCTGCAGC
Seq A exon
GTAAGCCCCAAGCTTCACAACCCCGCTTAGCTCTAACTGGGTTCTTCCATTCTGCCACCATTCATTCCAACCTCTTCCTTTCATCTGTTCTTCCGCAG
Seq C2 exon
AGGAGATCTCTGACCTGACCGAGCAGATTGCTGAGGGAGGGAAGCACATCCATGAGCTGGAGAAAATCAAGAAGCAAATTGATCAGGAAAAGAGTGAATTACAGGCTTCTCTGGAGGAAGCGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000057003:ENSMUST00000018632:31
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.339 A=NA C2=0.571
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(6.4=100),PF0003816=Filament=FE(17.8=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(4.8=100),PF0003816=Filament=FE(13.3=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGCTTGAAGCCTCCCAGAAG
R:
GCTCATGGATGTGCTTCCCTC
Band lengths:
181-279
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types