BtaINT0097295 @ bosTau6
Intron Retention
Gene
ENSBTAG00000037794 | MYH4
Description
myosin, heavy chain 4, skeletal muscle [Source:HGNC Symbol;Acc:HGNC:7574]
Coordinates
chr19:30085473-30085864:-
Coord C1 exon
chr19:30085699-30085864
Coord A exon
chr19:30085598-30085698
Coord C2 exon
chr19:30085473-30085597
Length
101 bp
Sequences
Splice sites
5' ss Seq
AGCGTGAGT
5' ss Score
8.2
3' ss Seq
GTTTTATTATTAAACTACAGAGG
3' ss Score
4.43
Exon sequences
Seq C1 exon
GTCCTATCAGAATGGAAACAGAAGTATGAGGAAACTCAGGCTGAGCTTGAGGCCTCCCAGAAGGAGTCCCGCTCTCTTAGCACTGAGCTGTTCAAAGTCAAGAACGCCTATGAGGAGTCCCTGGACCAACTTGAAACCCTAAAGAGAGAAAACAAGAACTTACAGC
Seq A exon
GTGAGTCTTTGTAGCGTTTCACTTTCAAAAATGGTAGACAGGTATCTTTTCAATTTTTCATCTTTCTCAACTGAACTTTGTGTTTTATTATTAAACTACAG
Seq C2 exon
AGGAGATTTCTGACCTGACCGAGCAAATTGCAGAGGGAGGAAAGCATATCCATGAACTGGAGAAAGTAAAGAAACAAATAGAACAGGAGAAGGGTGAACTACAGGCTGCTCTAGAGGAAGCAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000037794:ENSBTAT00000061014:30
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.357 A=NA C2=0.667
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(6.4=100),PF0003816=Filament=FE(18.6=100),PF045827=Reo_sigmaC=FE(38.7=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(4.8=100),PF0003816=Filament=FE(13.9=100),PF045827=Reo_sigmaC=PD(23.9=81.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTTGAGGCCTCCCAGAAGGA
R:
TCTGCTTCCTCTAGAGCAGCC
Band lengths:
245-346
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development