DreINT0173891 @ danRer10
Intron Retention
Gene
ENSDARG00000079564 | vmhc
Description
ventricular myosin heavy chain [Source:ZFIN;Acc:ZDB-GENE-991123-5]
Coordinates
chr2:24604692-24605184:-
Coord C1 exon
chr2:24605019-24605184
Coord A exon
chr2:24604817-24605018
Coord C2 exon
chr2:24604692-24604816
Length
202 bp
Sequences
Splice sites
5' ss Seq
AAGGTGAAT
5' ss Score
6.38
3' ss Seq
AAATGCGATGTTCTTTTCAGAGG
3' ss Score
6.27
Exon sequences
Seq C1 exon
GTACTGTCTGAGTGGAAGCAGAAGTTTGAAGAGTCGCAAGCCGAGTTAGAGAGCTCTCAGAAAGAAGCAAGATGTCTTAGCACTGAACTTTTCAAGCTGAAGAACTCCTATGAGGAAGCTTTAGATCACCTGGAGACCATGAAGAGAGAAAATAAAAATCTCCAAG
Seq A exon
GTGAATAAAAAGATTACCAACACAAAAACACTGTGTTTTTCTAAATCAACTAAAATAGATACATGAGGCAGACAGCAGAAGACTTTATACTGTGTTTACAATGATGGGGTGGAGGAAGCTTATAGTGCAGTACAGACATTCATTTTAAATTTTAGGATTGGAGAAAAAAAAATCAGTGTGTTAAATGCGATGTTCTTTTCAG
Seq C2 exon
AGGAGATTTCTGATCTCACCGAGCAACTTGGTGAGGGAGGAAAGAGCATCCATGAGCTGGAGAAAATGAGGAAACAGTTGGAGCAAGAAAAAAGTGAGATTCAATCTGCTCTGGAAGAGGCAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079564:ENSDART00000099532:31
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.393 A=NA C2=1.000
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(6.4=100),PF0003816=Filament=FE(16.5=100),PF045827=Reo_sigmaC=FE(39.3=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(4.8=100),PF0003816=Filament=FE(12.3=100),PF045827=Reo_sigmaC=PD(25.0=83.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGTCTGAGTGGAAGCAGAAGT
R:
CTCTGCCTCTTCCAGAGCAGA
Band lengths:
288-490
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]