MmuINT0103517 @ mm10
Intron Retention
Gene
ENSMUSG00000057003 | Myh4
Description
myosin, heavy polypeptide 4, skeletal muscle [Source:MGI Symbol;Acc:MGI:1339713]
Coordinates
chr11:67256187-67257295:+
Coord C1 exon
chr11:67256187-67256495
Coord A exon
chr11:67256496-67257091
Coord C2 exon
chr11:67257092-67257295
Length
596 bp
Sequences
Splice sites
5' ss Seq
AAGGTACCT
5' ss Score
8.49
3' ss Seq
TTTTTCTGCTTTCTGTGCAGGAC
3' ss Score
11.75
Exon sequences
Seq C1 exon
GCATCCCTTGAACATGAAGAAGGTAAAATCCTACGCATCCAGCTGGAGTTGAACCAGGTGAAATCTGAGATTGACCGCAAAATTGCTGAAAAAGATGAAGAGATAGATCAGCTGAAGAGAAACCACCTCAGAGTTGTGGAGTCCATGCAGAGCACGCTGGACGCCGAGATCCGCAGCAGGAATGATGCTCTGAGGATTAAGAAGAAGATGGAGGGAGACCTCAATGAGATGGAGATCCAGCTGAACCATGCCAACCGCCAGGCTGCGGAGGCAATCAGGAACCTTCGGAACACCCAGGGAATGCTGAAG
Seq A exon
GTACCTAGGGGTGAACATGCACTGTGTCAGCAGGCTCAGTGTTATAGAATTCAAATCTTAGATTTTTAGGAAAATTATAGGATATACATTACCAAAGCAATGTAGTATCAGGATAAAAAAAAATTGTATGTGCCTAGTTGGGAAGGAGCTATGTTCAGACTGAAAGGCCCAAGAGAGAATTTGCTTTGTCACCTGAGACAAAATGAGTGGTAAGGTAGATGTAGATACTGATGACACAATTCAGCTTCCCAAGTGCTAGGACTTAGGCACGACTCACCCAGCTCTGGACCTCCCCCTTTAAGCATCTAGAATGATGTAACCAGTGTAGCTGAATTCTAAGGGTACTTTCCTGCTACAAAATTGCACAGATTTATCCATGATTGATTTTTATGATTTTTGGGGATCTAAAATAAACCATTTTGTGCTTTTGAATTTAAGTACTGCACTAGGCATCCAAGAACAAGTCATTAAGGGTACTTGTAGATACTCCTATTTAATCAGACTCTGAGCTAATAAGATCCAGGTGTGAGCATGTCACTAAATTTGAATCCCTTTTAACTAGAGTGTGAGTAAGAGTTTTTCTGCTTTCTGTGCAG
Seq C2 exon
GACACACAGCTGCACCTGGACGATGCTCTCAGAGGCCAGGACGACCTGAAAGAGCAGCTGGCCATGGTTGAGCGCAGAGCCAACCTGATGCAGGCTGAGATCGAGGAGCTCAGGGCATCCCTGGAACAGACAGAGAGGAGCAGGAGAGTGGCCGAGCAAGAGCTACTGGATGCCAGTGAGCGCGTGCAGCTCCTCCACACCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000057003:ENSMUST00000018632:33
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.466 A=NA C2=0.662
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(11.9=100),PF0003816=Filament=PD(10.0=30.1)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(7.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTTGTGGAGTCCATGCAGAGC
R:
CTGGCATCCAGTAGCTCTTGC
Band lengths:
353-949
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types