MmuINT0103550 @ mm9
Intron Retention
Gene
ENSMUSG00000040752 | Myh6
Description
myosin, heavy polypeptide 6, cardiac muscle, alpha [Source:MGI Symbol;Acc:MGI:97255]
Coordinates
chr14:55582733-55584312:-
Coord C1 exon
chr14:55584097-55584312
Coord A exon
chr14:55582877-55584096
Coord C2 exon
chr14:55582733-55582876
Length
1220 bp
Sequences
Splice sites
5' ss Seq
AAGGTACGT
5' ss Score
10.75
3' ss Seq
CACCCACTCTGCTTCTCCAGACG
3' ss Score
8.2
Exon sequences
Seq C1 exon
AGTTTGAGTGACAGAATGACGGACGCCCAGATGGCTGACTTCGGGGCAGCAGCCCAGTACCTCCGAAAGTCAGAGAAGGAACGCCTAGAGGCCCAGACCCGGCCCTTTGACATCCGCACGGAGTGCTTCGTGCCTGATGACAAGGAGGAGTATGTTAAGGCCAAGGTCGTGTCCCGGGAAGGGGGCAAAGTCACTGCGGAAACTGAAAACGGAAAG
Seq A exon
GTACGTGCAATGGTGGCAAGACAGGGGAGGGCGGCTTAGGGAAAGGGGTTGGAGCAGAGCCTCCTGTAGCAGGAGCTGAGGAGCCTGAGAAGAGGTGGGGATGCCAAGATATGTTCCCTAGAGAACCTTCTCAGGGAAACCCAAGCAGGTGCACCAGGGTGTCACCCAAGGGATGCTCGCTTGCCAGAAAGACGCTGTCTTCCTCCTCAGAGAGGTCTTTATACAAAGGCACCAGCATAGCATTAAAGGCAGTTCCCCTTTCTAGCACTTGCATCCCTCCTGTCTGTGCCTCAGTTTCCCCCAAGAGTTCACTTTCTCAAATTCCCTTCTCCTAAACCAAGAGCAAATTCAGATCCAGATGAACAAATAGAGCAGAAATTTTTGAGCTGGGATCCGATTCCTGAGGCCAGTGATAGACACAGGAACCTCTGTTCCCTAGGTGCTCAGTCTAGCCGTGAGAACCACCCACCGCTCACCAGTAGTGCTCAGAGTTGTGTTCCTCACAGATTTGCTGCCTGGATGTTGCTTCCCCAGGATGTGTGCATTGATAGTCACAACCCACAAAGGATTATAATCTATCCCAGCTCAGTGAGCTCTGGTCACTATGAAGGCACAACATTTAACTGCTAAGAGCCACCACCTCTTCCACCATTAAAACAAAACAAAACAAAAAAACAAAAACAAAAAAACAAAAAACAAAAACCACTTGAATTCCAGTGGGCAGAAGCTATTGGCATCAAGACATAGAACACTGACACCAATCGACCCCCAATTTTGATGGATAAGGAGTTCAGGGTTGGGGTTGCTGCCTGTCCCACAGGGAAAAGGCTTTCCTGGAGCAACAGTGCTTGTGAGAACTATGTTCAGGAGACAGGCCCAGAGGCATGGCACAGCGTACATGGAATCTAGAGCTGCTAGTCCCCAGCTATGCCTTGGTCTGTTTAACACATGGGGGCAGAAAAGAGATGATCACTGGACAGGGTCAGGAGCTGGCATGGGGAAAGGAACTCTAGAACATCTCTAGAGCTCGGGCAGGAAAAATACCTGGGAACTGAGTCCTAGGGGATTTAGGGGCAGGAAACTGAAACCTTTGTCAGCCAAGAACTAGGGGAGCGAGGCCAAGCCAAGGACTAGGTAAGGGCATAAGGCTGGTAAAGGAGATCTGACAAGAGCCCATGGGAAGGTCTTCATCCATCCTATCACCCACTCTGCTTCTCCAG
Seq C2 exon
ACGGTGACCATAAAGGAGGACCAGGTGATGCAGCAGAACCCACCCAAGTTCGACAAGATCGAGGACATGGCCATGCTGACCTTCCTGCACGAGCCGGCTGTGCTGTACAACCTCAAGGAGCGCTACGCGGCCTGGATGATCTAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000040752-Myh6:NM_001164171:3
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.299 A=NA C2=0.333
Domain overlap (PFAM):
C1:
PF0273614=Myosin_N=PU(78.6=49.3),PF115253=CopK=PU(46.2=26.9)
A:
NA
C2:
PF0273614=Myosin_N=PD(16.7=14.6),PF0006316=Myosin_head=PU(4.1=58.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGTGACAGAATGACGGACGC
R:
ATAGATCATCCAGGCCGCGTA
Band lengths:
355-1575
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: