RnoINT0096509 @ rn6
Intron Retention
Gene
ENSRNOG00000025757 | Myh6
Description
myosin heavy chain 6 [Source:RGD Symbol;Acc:62029]
Coordinates
chr15:33627696-33629228:-
Coord C1 exon
chr15:33629011-33629228
Coord A exon
chr15:33627840-33629010
Coord C2 exon
chr15:33627696-33627839
Length
1171 bp
Sequences
Splice sites
5' ss Seq
AAGGTATGT
5' ss Score
9.79
3' ss Seq
CACCCACTCTCCTTCTCCAGACG
3' ss Score
9.99
Exon sequences
Seq C1 exon
AGTTTAAGAGTGACAGGATGACGGATGCCCAGATGGCTGACTTCGGGGCAGCAGCCCCATACCTCCGCAAGTCAGAGAAGGAGCGCCTAGAGGCCCAGACCCGGCCCTTTGACATCCGCACAGAGTGCTTCGTGCCTGATGACAAGGAGGAGTATGTCAAGGCCAAGATCGTGTCCCGGGAAGGGGGCAAGGTCACTGCCGAAACTGAAAACGGCAAG
Seq A exon
GTATGTGCAATGGTGGCAAGACAGGGGAGGGAGGGCGGCTTGGGGAAAGGGGTTGGAGCAAAGCCTCCTGTAGTGGGAGCTGAGGAGCCTGAGGAGAGGTGGGGATGCCAAGATGGTCCCTTGAAAACCCCAGGACCGGAACCGTTTCAGGGAGACCTAAGCGGGTGCACCAGGGGTCACCGAAGGGACGCTCTCCTGCCAGAAGGACACTGTCTTCCTCCTCAGAGAGGTCTTTAGATGAAGGCACCACCACAGAACTAAAGGCAGACGCCCTTTCTAGCACTTTCTATCCCTCCTGCCTGTGCCTCAGTTTCCCCCAAGAGTTCACTTTCTCAAATTCTCTTCTCCTAAACCAAAAGCAAACTCAGACCCAGATGAACAAAAAGGGCAGAAGCTTTTGAACCAAAAGCTGATTCCTGAGGCCAGCGATATACACAGGAGGGAACCCCTGTTCACTGGGTGCACAGCCTGGCCATGAGAATTACCCCCTGCTCGCCAGTAGGGCTCAGAGCTGTGTGACTCGCAGATTTGCTGCCTGGATGATGCTTCCCCCAGGATGTGACTGATCGCCACGACCCACAGGAATTATAATCTATCCCAGTTCAGCGAGCTCTGGTCACTATGAAGGCGCAACGTTTAACTGTTTAAAAAAAAAAAAAAAAAAAAAAAAAACTTCTTCCACTGTTAAAAAAAAAAAAAGAGCACTTGCATCCCTGTTGGCATCAAGACATAGGAAATGGATGGCAATCAACCCCCGATTTTGACAGATAAGGAGTACAGAGTTGGGGGTTTGCTGCTTGTCCCACTGGGGGAAAAGGCTTTCCTGGGGCAACAGAGGCACGCACAGGGCACGTGGATTTTAGAACTGCTAGTCTCTAGCTTTCCCTTTGTATGTTTAACTCATAGGGGCAGAAAAGAGATGATCACTGGACAGGATCAGGAGCTGGCAGGAGAAAGGAACCCTAGAACATCTCTGGAGCTTGGGCAGGAAAATTACCTGGGATTTGAGTCTAGTGGGGACTTAGGGCAGGAAGCTTAAACCTTTGTTAGCCAAGAACTAGGGGAGTGAGGCCAAGCAGGGAGGGGATAAGAGCACAAGGTTGGTAAATGAGATCTGACAAGAGCCATGGGAAGGTGGTCGCCCATCCTATCACCCACTCTCCTTCTCCAG
Seq C2 exon
ACGGTGACTGTGAAGGAGGACCAGGTGATGCAGCAGAACCCTCCGAAATTCGACAAGATCGAGGACATGGCCATGCTGACCTTCCTGCATGAGCCAGCTGTGCTCTACAATCTCAAGGAGCGCTATGCGGCCTGGATGATCTAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000025757:ENSRNOT00000023302:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.179 A=NA C2=0.250
Domain overlap (PFAM):
C1:
PF0273614=Myosin_N=PU(78.6=49.3)
A:
NA
C2:
PF0273614=Myosin_N=PD(16.7=14.6),PF0006316=Myosin_head=PU(4.1=58.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGTGACAGGATGACGGATGC
R:
GATCATCCAGGCCGCATAGC
Band lengths:
352-1523
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]