DreINT0100079 @ danRer10
Intron Retention
Gene
ENSDARG00000012944 | myhz2
Description
myosin, heavy polypeptide 2, fast muscle specific [Source:ZFIN;Acc:ZDB-GENE-020604-1]
Coordinates
chr5:31607655-31608138:+
Coord C1 exon
chr5:31607655-31607873
Coord A exon
chr5:31607874-31607994
Coord C2 exon
chr5:31607995-31608138
Length
121 bp
Sequences
Splice sites
5' ss Seq
ACGGTAATT
5' ss Score
7.31
3' ss Seq
CTTTTTATTTAAATTTATAGGAG
3' ss Score
8.09
Exon sequences
Seq C1 exon
TAAGCCGCCACCATGAGTACTGACGCGGAGATGGCCATTTATGGCAAGGCTGCCATTTTCCTCCGTAAGCCTGAGAAGGAGAGAATTGAAGCTCAGAGCAAACCATTTGATGCCAAAACCGCTTGTTATGTGGTTGATGACAAAGAGCTGTACGTCAAGGGAACAATCAAGAGCAAAGATGGTGGCAAAGTCACTGTTGTTACACTTGACACTCAGACG
Seq A exon
GTAATTTTACTCTTGCAATTCAAGCAGTTGTTATTTACACGCTAGAATGCTTTTTTAAAATTTTGTTTTAGTTATTTATTTTGCATTCATGTAAACACTTTCTTTTTATTTAAATTTATAG
Seq C2 exon
GAGAAAGTTGTTAAGGAGGATGATGTCCACCCAATGAATCCTCCCAAGTTTGACAAGATTGAGGACATGGCCATGATGACCCATCTCAATGAACCCTCTGTGCTGTATAACCTCAAAGAGCGTTATGCAGCATGGATGATCTAC
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000012944:ENSDART00000126873:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.029 A=NA C2=0.125
Domain overlap (PFAM):
C1:
PF0273614=Myosin_N=PU(79.1=49.3)
A:
NA
C2:
PF0273614=Myosin_N=PD(16.3=14.6),PF0006316=Myosin_head=PU(4.1=58.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCCTCCGTAAGCCTGAGAAGG
R:
GGTCATCATGGCCATGTCCTC
Band lengths:
242-363
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]