RnoINT0096267 @ rn6
Intron Retention
Gene
ENSRNOG00000049695 | Myh1
Description
myosin heavy chain 1 [Source:RGD Symbol;Acc:735061]
Coordinates
chr10:53713938-53714393:+
Coord C1 exon
chr10:53713938-53714144
Coord A exon
chr10:53714145-53714249
Coord C2 exon
chr10:53714250-53714393
Length
105 bp
Sequences
Splice sites
5' ss Seq
GCGGTGTGT
5' ss Score
5.95
3' ss Seq
TTATCTTTCCTGTTCACCAGACC
3' ss Score
9.03
Exon sequences
Seq C1 exon
GCCATGAGTTCCGACTCTGAGATGGCCGTTTTCGGGGAGGCTGCTCCTTACCTCCGGAAGTCTGAAAAGGAGCGAATCGAGGCCCAGAATAGGCCTTTTGATGCCAAAACATCTGTCTTTGTGGCGGAGCCCAAGGAGTCCTTTGTCAAAGGAACCATTCAGAGCAAAGACGCAGGGAAAGTGACTGTGAAAACTGAAGCAGGAGCG
Seq A exon
GTGTGTAGAGGGCCTGCCATGTTGTACCCGTTACATTTGTGCTGCATTTTGCAGGGTTGATCATTCATTCACTCGACACCAAAAATTATCTTTCCTGTTCACCAG
Seq C2 exon
ACCCTGACGGTGAAAGAAGACCAGATCTTCCCCATGAACCCTCCCAAGTACGACAAGATCGAGGACATGGCCATGATGACCCACCTCCACGAGCCCGCTGTGCTGTACAACCTCAAAGAGCGTTACGCAGCCTGGATGATCTAC
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000049695:ENSRNOT00000004236:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.058 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0273614=Myosin_N=PU(78.6=47.8)
A:
NA
C2:
PF0273614=Myosin_N=PD(16.7=14.6),PF0006316=Myosin_head=PU(6.2=58.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTCCTTACCTCCGGAAGTCT
R:
GTCATCATGGCCATGTCCTCG
Band lengths:
245-350
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]