BtaINT0097275 @ bosTau6
Intron Retention
Gene
ENSBTAG00000007090 | MYH2
Description
Bos taurus myosin, heavy chain 2, skeletal muscle, adult (MYH2), mRNA. [Source:RefSeq mRNA;Acc:NM_001166227]
Coordinates
chr19:30162878-30163333:-
Coord C1 exon
chr19:30163110-30163333
Coord A exon
chr19:30163022-30163109
Coord C2 exon
chr19:30162878-30163021
Length
88 bp
Sequences
Splice sites
5' ss Seq
GCGGTGAGT
5' ss Score
10.49
3' ss Seq
TTATCCTTCCTGTTTACCAGACT
3' ss Score
7.95
Exon sequences
Seq C1 exon
GACCTCTGAGTTCAGCAGCCATGAGTTCGGACCAGGAAATGGCCATCTTTGGGGAGGCTGCTCCTTACCTCCGAAAGTCTGAAAAGGAGCGCATTGAAGCCCAGAATAAGCCTTTTGATGCCAAGACATCTGTCTTTGTGGCCGAGCCCAAGGAATCTTTTGTCAAAGGGACTATCCAGAGCAGAGAAGGTGGGAAAGTGACAGTGAAGACCGAAGGAGGAGCG
Seq A exon
GTGAGTAGTCCAGGAGCTGGCCACATGCTACTGATTATTTTGTTTGGCCACTTGGTTGACATAAGCAGTTATCCTTCCTGTTTACCAG
Seq C2 exon
ACTCTGACAGTGAAAGAGGATCAAGTCTTCCCCATGAATCCTCCCAAATTCGACAAGATCGAGGACATGGCCATGATGACCCACTTGCACGAGCCTGCTGTGCTGTACAACCTCAAAGAGCGTTATGCAGCCTGGATGATCTAC
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000007090:ENSBTAT00000012797:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.191 A=NA C2=0.042
Domain overlap (PFAM):
C1:
PF0273614=Myosin_N=PU(78.6=48.5)
A:
NA
C2:
PF0273614=Myosin_N=PD(16.7=14.6),PF0006316=Myosin_head=PU(4.1=58.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCCGAGCCCAAGGAATCTTTT
R:
TCGTGCAAGTGGGTCATCATG
Band lengths:
176-264
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]