Special

MmuINT0103592 @ mm10

Intron Retention

Gene
ENSMUSG00000053093 | Myh7
Description
myosin, heavy polypeptide 7, cardiac muscle, beta [Source:MGI Symbol;Acc:MGI:2155600]
Coordinates
chr14:54974642-54975077:-
Coord C1 exon
chr14:54974912-54975077
Coord A exon
chr14:54974767-54974911
Coord C2 exon
chr14:54974642-54974766
Length
145 bp
Sequences
Splice sites
5' ss Seq
AGGGTGGGC
5' ss Score
4.44
3' ss Seq
GTCACTGTGTGCCCCCACAGAGG
3' ss Score
8.84
Exon sequences
Seq C1 exon
ATCCTGGCTGAGTGGAAGCAGAAGTATGAGGAGTCGCAGTCAGAGCTGGAGTCTTCCCAGAAGGAGGCGCGCTCCCTGAGCACAGAGCTCTTCAAGCTCAAGAACGCCTATGAGGAGTCTCTGGAGCACCTAGAGACCTTCAAGCGGGAGAACAAGAACCTCCAGG
Seq A exon
GTGGGCGAGCTGGGGGACAGGAAAGGGCTGCAGGGAAAGGGAAGGGCCATGTCCCCATCTCCAATAGCAGTCCAGGGAAAGAAGCACACCCCTACTCTATTGAGACCCCAGAAATTCCTGACTGAGTCACTGTGTGCCCCCACAG
Seq C2 exon
AGGAGATCTCAGACCTGACTGAGCAGCTGGGCTCCACGGGGAAGAGCATCCATGAGCTGGAGAAGATCCGAAAGCAACTGGAGGCCGAGAAGCTGGAGCTGCAGTCGGCCCTGGAGGAGGCTGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000053093:ENSMUST00000102803:33
Average complexity
IR
Mappability confidence:
NA
Protein Impact





	

	
ORF disruption upon sequence inclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.393 A=NA C2=0.595
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0157614=Myosin_tail_1=FE(6.4=100),PF0003816=Filament=FE(17.9=100),PF045827=Reo_sigmaC=FE(37.4=100)

							
A:
NA

							
C2:
PF0157614=Myosin_tail_1=FE(4.8=100),PF0003816=Filament=FE(13.3=100),PF045827=Reo_sigmaC=FE(27.9=100)

						

					

				








    
Main Inclusion Isoform:
NA





     
                   









    
Main Skipping Isoform:
ENSMUSP00000099867





     
      









    
Other Inclusion Isoforms:
NA
          









    
Other Skipping Isoforms:
ENSMUSP00000126840
  



Associated events






Other assemblies


mm9




Conservation


Human
(hg38)
chr14:23416993-23417152 
ALTERNATIVE
HsaINT0107861
(MYH7)
Human
(hg19)
chr14:23886202-23886361 
LOW PSI
HsaINT0107861
(MYH7)
Rat
(rn6)
chr15:33638654-33638739 
Cow
(bosTau6)
chr10:21341566-21341690 
Chicken
(galGal4)
chr18:622105-622276 
Chicken
(galGal3)
chr18:358953-359012 
GgaINT0127800
([1])
Zebrafish
(danRer10)
chr24:41065074-41065153 
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
CTGGCTGAGTGGAAGCAGAAG

				
R: 
GCCTCCAGTTGCTTTCGGATC

				
Band lengths: 
248-393

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Ribosome-engaged transcriptomes of neuronal types
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types





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