Special

MmuINT0103666 @ mm9

Intron Retention

Gene
ENSMUSG00000055775 | Myh8
Description
myosin, heavy polypeptide 8, skeletal muscle, perinatal [Source:MGI Symbol;Acc:MGI:1339712]
Coordinates
chr11:67113215-67113653:+
Coord C1 exon
chr11:67113215-67113333
Coord A exon
chr11:67113334-67113456
Coord C2 exon
chr11:67113457-67113653
Length
123 bp
Sequences
Splice sites
5' ss Seq
AAGGTGAGT
5' ss Score
10.47
3' ss Seq
CTGGACTATCATTTCTACAGGCC
3' ss Score
7.06
Exon sequences
Seq C1 exon
GTGAGTACTCCAGACAGCTGGATGAGAAAGATGCTTTAGTCTCTCAGTTGTCAAGGAGCAAGCAGGCATCCACGCAGCAGATTGAGGAGCTGAAGCGTCAGCTCGAGGAAGAAACCAAG
Seq A exon
GTGAGTTTTACCCGAGATCTCGACAAACCAAACCCGAGAATGCCCAGCTATAGCTACTTGAGGTGTGTGAGTTAAGTGTTACAGGACATACAAATTGAAATCCCTGGACTATCATTTCTACAG
Seq C2 exon
GCCAAGAACGCGCTGGCCCACGCCCTGCAGTCCTCCCGCCACGACTGTGATCTGCTGCGGGAACAGTATGAAGAGGAGCAGGAAGGCAAAGCCGAGCTGCAGAGGGCACTGTCCAAGGCCAACAGCGAGGTGGCCCAGTGGAGGACCAAATATGAGACCGATGCCATCCAGCGCACAGAGGAGCTGGAGGAGGCCAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000055775-Myh8:NM_177369:29
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact





	

	
ORF disruption upon sequence inclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.875 A=NA C2=0.485
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0003816=Filament=PD(4.9=35.0),PF0157614=Myosin_tail_1=FE(4.5=100),PF0003816=Filament=FE(41.9=100),PF0003816=Filament=PU(10.8=77.5)

							
A:
NA

							
C2:
PF0157614=Myosin_tail_1=FE(7.6=100),PF0003816=Filament=PD(30.1=42.4),PF0003816=Filament=FE(22.7=100)

						

					

				








    
Main Inclusion Isoform:
NA





     
                   









    
Main Skipping Isoform:
ENSMUSP00000019625





     
      









    
Other Inclusion Isoforms:
NA
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr17:10398641-10398767 
LOW PSI
HsaINT1024149
(MYH8)
Human
(hg19)
chr17:10301958-10302084 
HsaINT0107940
(MYH8)
Rat
(rn6)
chr10:53840186-53917277 
LOW PSI
RnoINT0096607
(Myh8)
Cow
(bosTau6)
chr19:30039384-30039507 
LOW PSI
BtaINT0097485
(MYHC-FETAL)
Chicken
(galGal4)
chr18:357371-357690 
LOW PSI
GgaINT0128204
([1])
Chicken
(galGal3)
chr18:360063-360382 
LOW PSI
GgaINT0128204
([2])
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
AGTTGTCAAGGAGCAAGCAGG

				
R: 
GCTGGATGGCATCGGTCTCAT

				
Band lengths: 
246-369

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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