MmuINT0115833 @ mm10
Intron Retention
Gene
ENSMUSG00000031860 | Pbx4
Description
pre B cell leukemia homeobox 4 [Source:MGI Symbol;Acc:MGI:1931321]
Coordinates
chr8:69870578-69872292:+
Coord C1 exon
chr8:69870578-69870684
Coord A exon
chr8:69870685-69871994
Coord C2 exon
chr8:69871995-69872292
Length
1310 bp
Sequences
Splice sites
5' ss Seq
CTGGTGAGT
5' ss Score
10.1
3' ss Seq
TCATGCTCTTATCTTTCCAGGTC
3' ss Score
11.6
Exon sequences
Seq C1 exon
GTCCCTGTGGCCCCTTGCCACTGACCAATGGAAGTGACGTGGTTCTCACTCTGCGGACCCTGGCCTTCCTCCAGCCCCCCACTGGGGGAGTCTGCCTACAGCCCCTG
Seq A exon
GTGAGTCAGCTGGGACCCCTCCAGGGCTTTGGGGAGGTAGAGGAGCCCTGGTGTTGGGTCTCTGGGGAAGGAAACCCCAAGGAAAGGTGGGAGCATTTAACCTCTGGACTCAGACCCACCTGTCACGGGGGCTGGAGACCTGGTGGAGTGGGGGTGGCTCACACTGAAAAGGCATCTCTGCCAGGCTGGGATACACGTTAGTAACTGGGGTCTAGACAGCCAAGTTGCCTCCAGAACTTTGCCAGGGTGGCAGCTGTGTAGAGCCTGTGACCTCTCAGTCTGTCCTGGGTGTCGGATGAGGGCCCTGCATAAACAGAGTGGGCTCTGGCATGGTGGCCCCATGAGCCCAGCAGCTTCCCTGAAGCTCCCTTAGAACCAGCCCCGGGGCTTGGAGTAGTGCCCACCGGGAGTGAGCTGGGTGAGCCCGGCTCTCTCAGGCCCTGCCTGCCACCACCTTGAGTCCCAGCCTAATGCAAGCTTCAGGCTGTGGAGCAGAGTCTGGGCCTGCTTCTCGAACACCTGAAGCTAAGGTGCTCTGGGACAGAGCCAGCAGGAGTGAGGGAGCGAAGCACCAGTCTGCCCTGCTCTGCCACACCTGCCCATTCCAGCACTGAGCCAAGAGTCAGGCTGTGCCATGCCCTGCAGGAACCCACCTAAGTGTCCTGTGGGTACAACATGTCCCAAGGACACCCTTTGTCTTGTTGTTTGTTTAAGATGATCTCTCTACATACCACTGGCTGTCCTGGTATTCACTCTTTAGACCAGGCTAGCCTTGAATTCACAGAGTTCTTCCTGCCTCTGTCTCCCAAGGGCTTAAAGGAATATACCATCATGACAGACCCATGCCAACACCTTCATACCCATTCAGCTGGCTCTTCTTAAAGGTCACTCTGCCAGCATGGCCACTGTTCTTCTATCCAGCTGCTTCAGCATATGCCTGCCATGGCCTCACTCACAGAGCCTCTAGCACACATGCCATGCCTGATACAGTGGACAAGGACAGTGTCCTTTGAGCTTAGCACATCCATCCAGCTGTCCTTAGACAGATGGGCAGAGTAGCCCCAATGTGTGCTGTGGTCATGCACACTGCAGACAAGCGGAGGCTCCTGCCCCTGTGAGGAAGGAGCCCACAGTCATGGCCTTGCCACAGGCTGCTGGTTCTGACATCCCCCAAGGCATTTGCTGGAGGGGTGTGGGCTCAGAGCCCACCTGGCTCTTTCTGGAACTCCTCTGCTAGCATGTTCTGTGTAGGTACCTTTGGGGACACAGGAACTGGGTAGTCACCCACTCTCATGCTCTTATCTTTCCAG
Seq C2 exon
GTCCATAGTAACTGGCAGAGGGCCGCCCCACAGCCAGCCTCATCACCTGCGGGAGAGTCTGGCAGCTTCAATTGGGATGCTGCATCTAATTAAGTTTCTGGCACCTGGCTGAGTGCCATAGGTGACTGGCCCTGTGTCCTGCTGATGACCTCAGAAGTCCTGGCTGCGGCTTTGCCCTTTTTCAGGCCACTTGCCTCCCCACCTTGGTGTGATTTTTTTTTTTAAAGAAACATCAGTTAAGTGACTGACAGTGGACTGTTCATTCTGAGCACCCGATTAAAGTTTTGACCTGTTTGCA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000031860:ENSMUST00000134777:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.257 A=NA C2=0.867
Domain overlap (PFAM):
C1:
PF037928=PBC=PD(3.5=5.4)
A:
NA
C2:
NO
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGACGTGGTTCTCACTCTGC
R:
CGGGTGCTCAGAATGAACAGT
Band lengths:
349-1659
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types