MmuINT0117036 @ mm10
Intron Retention
Gene
ENSMUSG00000024575 | Pde6a
Description
phosphodiesterase 6A, cGMP-specific, rod, alpha [Source:MGI Symbol;Acc:MGI:97524]
Coordinates
chr18:61264173-61265065:+
Coord C1 exon
chr18:61264173-61264273
Coord A exon
chr18:61264274-61264957
Coord C2 exon
chr18:61264958-61265065
Length
684 bp
Sequences
Splice sites
5' ss Seq
CAAGTGGGT
5' ss Score
4.82
3' ss Seq
CAAACCCGTATGTCTTTCAGGAA
3' ss Score
8.33
Exon sequences
Seq C1 exon
AGCCTGAATATCTTCCAGAACCTGAATCGCCGGCAGCATGAGCACGCGATCCACATGATGGACATCGCGATCATTGCCACAGACCTTGCCTTGTATTTCAA
Seq A exon
GTGGGTATTTCTCCTCACTTTAATAGTAGCAGTGTGGGGGCTGGAGAGATGGTTCAGTGGTTAACAGCACTGACTGCTCTTCCAGAGGTCCTGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACTATCTGTAATGGGATCTGATACCCTCTTCTGGTGTGTGTCTGAAGACAGCGATGGAGTACTCACATATATAAATAAATAATAAATAAATAAATAAATCTTTAATAGTACCAATGGCTATAGTAGCAAATGCAGTAGTTCCTGCTTATCTTTGTTCTGCTTCAGGTCCAACACATCGCCCTTGTAAGATAGATGCTCTTACACCCCATCCTAGAGGGAAACAGATTTCCTAAACACATAGATCAGTGGCATCTAGTCTAGAACCTAGTCTTTATTTCAAGGCTGGAGGGTTTTTCCAGGTTGTTTTGTTAACTATGTGAATTTAATAATGTCCCCAACTGAAACAGCCCATGTTTTGGGAGGGTGGGGCAGTCAAGACAGAGTTTCTGTGCAGCCCTGGCTGTACTGGAACTCACTCTATAGACCAGGCTGGCCTCTCACTCAGAGATCTGCCTGCCTCTGCCTCTTGAGTGCTGGGATGAAAGGCGTGCACCACCCCTGAGCTGCTTGCTACCCCTTTGTACTTGACCTCTTTACCAAACCCGTATGTCTTTCAG
Seq C2 exon
GAAAAGGACCATGTTCCAGAAGATTGTGGATCAGTCAAAGACATATGAGAGTACCCAGGAGTGGACCCAGTACATGATGCTGGAGCAGACACGGAAGGAAATTGTGAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000024575:ENSMUST00000025468:16
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0023314=PDEase_I=FE(13.3=100)
A:
NA
C2:
PF0023314=PDEase_I=FE(14.5=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGCCTGAATATCTTCCAGAACCTG
R:
ACAATTTCCTTCCGTGTCTGCT
Band lengths:
206-890
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types