MmuINT0117043 @ mm9
Intron Retention
Gene
ENSMUSG00000024575 | Pde6a
Description
phosphodiesterase 6A, cGMP-specific, rod, alpha [Source:MGI Symbol;Acc:MGI:97524]
Coordinates
chr18:61392486-61394433:+
Coord C1 exon
chr18:61392486-61392575
Coord A exon
chr18:61392576-61394292
Coord C2 exon
chr18:61394293-61394433
Length
1717 bp
Sequences
Splice sites
5' ss Seq
CAGGTAAGT
5' ss Score
10.86
3' ss Seq
CCTTCTTCTTCTGTCTTCAGATA
3' ss Score
12.13
Exon sequences
Seq C1 exon
ATTCTTCTCAAGTACCTCAACTTTGTGAACCTGATCATGAAGGTATTCCACCTGAGCTACCTGCACAACTGTGAGACTCGTCGCGGCCAG
Seq A exon
GTAAGTCGCGGCCTGGCTCCAGAACGGGAGTGGGAGTGGGGGTGGGGGTGGGGGTGGGGGTGGAGGGGTGATGAGCATGTCCTTCTACTGAAGCATTGACAGGGTCAGGGTGGCTGGTGTGAAGGTGCTGGGGACTACTGGGCTGTGTCAGGTGGGAGACCCCAGTATTAGAAGGTGATAAATGCTTGTGTCTCAGTCAGAGTTTCTATTCCTGCACAAACATCATGACCAAGAAGCAAGTTGGGGAGGGAAGAGTTTATTCAGCTTACACTTCCATGTTGCTGTTCATCACCAAAGGAAGTCAGGACTGGAACTCAAGCAGGTCAGGAAGCAGGAGCTGATGCAGAGGCCATGGAGGGATGTTTCTTACTGGCTTGCTTCCCCTGGCTTACTCAGCATGCTTTCTTATAGAACCTAAGACTACCAGTCCAGAGATGGTACCACCCACAAGGGGCCCTTCCCCTCTTGATCACTAATTGAGAAAATGCCTTACAGCTGGATCTCATGGAGGCACTTCCCCAGTTGATAACTCCTGCCTGTGTCAAGTTGACACACAAACCCAGCCAGCACAGCTTGTGAGGTTGTATCCTAGAGCGGTTATGGCTGGACTTGAACACTTGTTCACTAAGGAGCTGTCAGACCCTCAGCAAGTTTCTTAGCCTCTCTAAGGTGAAGATAGGCAGGGTGGTGCTTGCTTGTCTTCCCAGAACTCAGGAGGTGGAGGCAGCAGCATCAGGAGTTCAAGGTCAGCCTCAACTTATAGCTAAGTTGAGGCCCACTGAGCGACACGAGACCAGATACAAACAAACAAAGAAACAAACAAGAACATGTGAATAATTGTATATCACTCTCAAAGATTGTTATCAAAATAAATAAATTAACATATGCCAGGTACTTATGCTCAGCTGCTTGTTATAATTTGATTTTATGTTTTTTTGAGGTGGTGGGGATTGAACTCATAGCCTGTACCTATAAGGCAAGCACTCGGCTATGGAGCTGCTCTCCTAAACCCTTTGCTTGCTTTAGTAACCACATATGAAACAAAATAAACGGAAAGAATTAGGCCCAATGTAAGCCAGCTGATCACTTCACCTCTCCAAGCCTCAGTTTTCCACCTATAAAATGGGGCTAATGACCCCATTTGTTTCTCTAGATCAGTGGTTCTCAACCTTCCTGATACCACAACACTTTAATGCAGGTCCTCAAACTGGAGTGACCGCCCCCCTCCCCAGCCATAATATAAGCCTCATTGCTACTCCATAACTGTAATTTTGCTACTGTTATGAATCAGAATGTAAATATCTGTATTGTCCTATGGTCTTAGGTGACCCCTGTGAAAGGGTCACTTGACCCCCACAGGGGTTGCAACCTACAGGCTGAGAACCACTGTTCTAGATTATTATGAGACTTAAATAGATTAAGTAGAAAACTCTGCAGAATTGTTATTACTTTCTACCTAAGAAACTTCTAAGTTGTACTTCAAAAACTCGGATTGTGGGGGCAGGGACTTGTGAGGGCGGGACTGGGAGGAGAGGAGGGAAGGGGCTGTAATCGAGATATAAAGTGAATACAAAAATAAATTATTGGAAAAAAAACCTCTGTTTTCTTCTTCTTCTTCTTCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCTTCCTCCTCCTCCTCCTCCTTCTTCTCCTTCTCCTTCTTCTTCTGTCTTCAG
Seq C2 exon
ATATTGCTGTGGTCTGGGAGCAAGGTCTTTGAGGAGCTCACGGATATAGAGAGGCAGTTCCACAAGGCCCTGTACACGGTCCGGGCTTTCCTCAACTGTGACAGATACTCCGTAGGACTCTTAGACATGACCAAACAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000024575-Pde6a:NM_146086:3
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0159021=GAF=PD(7.3=40.0)
A:
NA
C2:
PF0159021=GAF=PU(18.0=68.1)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATTCTTCTCAAGTACCTCAACTTTGT
R:
TTCTGTTTGGTCATGTCTAAGAGTCC
Band lengths:
230-1947
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: