MmuINT0117397 @ mm9
Intron Retention
Gene
ENSMUSG00000019577 | Pdk4
Description
pyruvate dehydrogenase kinase, isoenzyme 4 [Source:MGI Symbol;Acc:MGI:1351481]
Coordinates
chr6:5441591-5442014:-
Coord C1 exon
chr6:5441830-5442014
Coord A exon
chr6:5441678-5441829
Coord C2 exon
chr6:5441591-5441677
Length
152 bp
Sequences
Splice sites
5' ss Seq
ACAGTAAGT
5' ss Score
9.49
3' ss Seq
TTGTTTTCATTTTTCAACAGTCC
3' ss Score
9.32
Exon sequences
Seq C1 exon
GTTTGTAGACACGCTGGTCAAAGTTCGAAACAGACATCATAATGTGGTCCCTACAATGGCTCAAGGCATCCTGGAGTATAAAGACACCTGCACAGTGGACCCCGTTACCAATCAAAATCTTCAGTATTTTTTAGACCGGTTTTACATGAACCGCATTTCTACTCGGATGCTCATGAATCAGCACA
Seq A exon
GTAAGTGAACCTCTCCATGCATGGCCCAGGCATGGGTACTGAGCACAAGCTACGCTGACGCAGGGCCCGAGACTGGGCACTCACGTGGTTTGTGGTAGCTCTAGTCACCACCCTGAGTTTTATAACATGCTTTTGTTTTCATTTTTCAACAG
Seq C2 exon
TCCTCATATTCAGTGACTCAAAGACGGGAAACCCAAGCCACATTGGAAGTATCGACCCAAACTGTGATGTGGTAGCAGTAGTCCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000019577-Pdk4:NM_013743:4
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF104364=BCDHK_Adom3=FE(37.8=100),PF0160215=Adaptin_N=PD(43.8=61.9)
A:
NA
C2:
PF104364=BCDHK_Adom3=PD(11.6=63.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GACACGCTGGTCAAAGTTCGA
R:
ACCACATCACAGTTTGGGTCG
Band lengths:
251-403
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: