MmuINT0119671 @ mm10
Intron Retention
Gene
ENSMUSG00000030660 | Pik3c2a
Description
phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide [Source:MGI Symbol;Acc:MGI:1203729]
Coordinates
chr7:116376223-116376559:-
Coord C1 exon
chr7:116376514-116376559
Coord A exon
chr7:116376434-116376513
Coord C2 exon
chr7:116376223-116376433
Length
80 bp
Sequences
Splice sites
5' ss Seq
AGGGTATGT
5' ss Score
8.34
3' ss Seq
AAGACTCTGTTTCAATGTAGGGT
3' ss Score
6.66
Exon sequences
Seq C1 exon
GTGACTTCATTATCTGGAAGTGACACAAGGAAGAACTCAACTAAGG
Seq A exon
GTATGTCTTTAGATTTTTTGTTTTACATTTTGGCAATGATTTTTGCTTTTTCAGAGAATTAAGACTCTGTTTCAATGTAG
Seq C2 exon
GGTCACTGAATCCTGAAAATCCTGTTCAAGTAAGCATGGATCACCTAACAACAGCGATTTATGATCTTCTCAGGCTCCATGCAAATTCTAGTAGGTGTTCTACAGGCTGTCCCCGAGGGAGCAGGAACATCAAGGAAGCATGGACTGCAACGGAGCAGCTCCAGTTCACTGTCTATGCCGCACACGGAATTTCCAGTAACTGGGTATCAAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000030660:ENSMUST00000170430:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=NA C2=0.127
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0079219=PI3K_C2=PU(0.1=0.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCTGGAAGTGACACAAGGAAGA
R:
TCCTTGATGTTCCTGCTCCCT
Band lengths:
170-250
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types