MmuINT0120834 @ mm10
Intron Retention
Gene
ENSMUSG00000004591 | Pkn2
Description
protein kinase N2 [Source:MGI Symbol;Acc:MGI:109211]
Coordinates
chr3:142811527-142812057:-
Coord C1 exon
chr3:142811914-142812057
Coord A exon
chr3:142811603-142811913
Coord C2 exon
chr3:142811527-142811602
Length
311 bp
Sequences
Splice sites
5' ss Seq
GAGGTAATA
5' ss Score
7.96
3' ss Seq
TTGTATTTTCTTTATTGCAGGTT
3' ss Score
10.97
Exon sequences
Seq C1 exon
TCACGTGAACTGGAAATTTCAGTTTATTGGCGTGATTGGCGATCTCTGTGTGCTGTAAAATTTCTGAGGTTAGAAGATTTTTTAGACAACCAGCGGCATGGCATGTGTCTCTATTTGGAACCACAGGGTACTTTGTTTGCAGAG
Seq A exon
GTAATACATGTATTTTATTAATGTGCATAACCACAGTAGAAATTGTATGTGTTATCAGCATGATGTAGAAATGGCAAGAGTGTTAGGCTGAGAGACAGATATGTTGAGCTCTTGGCTGCACTGCTGCATAAGGACGTGACCTTGAGGAAACTCATGTCTCCTCTAGGTCTGTGTTCTCATCTGGAAAAGGAAGGGGTTGGACTGATTGTAGCTAAGGTGCCTTCACGTTCTCCAGCTTAGGAATCTAGTAGATTTTAGTGAATCATAGTCCATTACAGTGTTGATAGCAACTTGTATTTTCTTTATTGCAG
Seq C2 exon
GTTACTTTTTTTAATCCAGTTATTGAAAGACGACCAAAACTCCAAAGACAAAAGAAAATTTTTTCAAAACAACAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000004591:ENSMUST00000043812:9
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.082
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCACGTGAACTGGAAATTTCAGT
R:
CTTTTGTCTTTGGAGTTTTGGTCGT
Band lengths:
198-509
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types