MmuINT0131352 @ mm9
Intron Retention
Gene
ENSMUSG00000026821 | Ralgds
Description
ral guanine nucleotide dissociation stimulator [Source:MGI Symbol;Acc:MGI:107485]
Coordinates
chr2:28399074-28399906:+
Coord C1 exon
chr2:28399074-28399267
Coord A exon
chr2:28399268-28399508
Coord C2 exon
chr2:28399509-28399906
Length
241 bp
Sequences
Splice sites
5' ss Seq
AGGGTGAGG
5' ss Score
7.13
3' ss Seq
GGGCATCTTATCTCCTGCAGCCC
3' ss Score
7.41
Exon sequences
Seq C1 exon
TGCCATCTCCTCCATCCTGGGCACCTGGCTGGACCAATACTCAGAGGATTTCTGTCAACCTCCGGACTTTCCCTGCCTCAAGCAGCTGGTGGCTTATGTACAGCTCAACATGCCTGGCTCAGATCTGGAGCGCCGCGCTCACCTTCTCCTGGCCCAGCTGGAGGACCTGGAGCCCAGTGAGGCTGAGTCTGAGG
Seq A exon
GTGAGGAACCAGGGGTAGCCACACAGAGCCTGCTAGGAGGGTCAGTAAAAGTTTCTACCACAGAGAGGCTCCAGCCACCGAGCCAGGAGCAAGAGCAAGCCTGGGGCCCAGTAGGTAGTACACCAGAGTCTGGGATACCATCTTGTTGTTGGGATGGGGGCTTGTTCTCTGGCAGTCGGTGATTCTCGGTCTTGAAATGCTCTGGACAAGGCAGCCATAGTGGGCATCTTATCTCCTGCAG
Seq C2 exon
CCCTGTCCCCAGCTCCAGTGCTGTCTCTGAAGCCAGCTTCACAGCTAGAACCTGCACTGCTGCTGACGCCCAGCCAAGTGGTGACATCAACTCCAGTACGAGAGCCCGCTGCGGCCCCAGTGCCAGTGCTGGCCTCCAGCCCAGTGGTGGCACCAGCTCCTGAGCTAGAACCAGTTCCAGAGCCACCTCAAGAGCCTGAGCCATCCCTAGCACTGGCTCCAGAGCTGGAGCCCGCCGTCTCACAGAGCCTGGAACTGGAGTCAGCTCCTGTGCCCACTCCTGCCTTAGAGCCTTCCTGGTCTCTGCCTGAAGCCACGGAGAATGGACTAACCGAGAAGCCTCACCTTCTGCTGTTCCCTCCTGACTTGGTGGCTGAACAGTTTACTCTGATGGATGCA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000026821-Ralgds:NM_001145835:5
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.147 A=NA C2=0.601
Domain overlap (PFAM):
C1:
PF0061815=RasGEF_N=PD(28.3=48.5)
A:
NA
C2:
PF0061714=RasGEF=PU(7.4=12.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGTACAGCTCAACATGCCTGG
R:
CTAGGGATGGCTCAGGCTCTT
Band lengths:
308-549
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: