MmuINT0131354 @ mm10
Intron Retention
Gene
ENSMUSG00000026821 | Ralgds
Description
ral guanine nucleotide dissociation stimulator [Source:MGI Symbol;Acc:MGI:107485]
Coordinates
chr2:28545010-28545518:+
Coord C1 exon
chr2:28545010-28545225
Coord A exon
chr2:28545226-28545414
Coord C2 exon
chr2:28545415-28545518
Length
189 bp
Sequences
Splice sites
5' ss Seq
AGGGTAAGC
5' ss Score
9.04
3' ss Seq
CACAGACTCCTTGTCCCCAGGAG
3' ss Score
6.82
Exon sequences
Seq C1 exon
GAACTATTCAAGAAAGTCGTGCCCTACCACTGCCTGGGCTCCATCTGGTCCCAACGGGACAAGAAGGGCAAGGAGCACCTCGCGCCTACCATCCGCGCCACTGTCGCCCAGTTCAACAACGTGGCCAACTGTGTCATTACTACCTGCCTTGGGGACCAGAGTATGAAGGCTCCGGACAGGGCCCGGGTGGTGGAACACTGGATCGAGGTGGCCAGG
Seq A exon
GTAAGCTACAGGAGGGCCTCTTCTTTTTCTTCATAGACCTCAGGGCCACACAGCCAGGTGTATACTCGCTCCAGGCACTGTACACTTGAGTCGCTGTGTGAACATGTGTCCCTGGCATGCCCTCTGGGAGCTTCCTGGCATCAGGAAGGGCTCATCCATGGGCTCCACTCACAGACTCCTTGTCCCCAG
Seq C2 exon
GAGTGCAGAGCGCTCAAGAATTTCTCCTCCCTCTACGCCATCCTCTCTGCTCTACAGAGCAATGCCATCCACCGCCTAAAGAAGACGTGGGAAGAGGTCTCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000026821:ENSMUST00000028170:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.014 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0061714=RasGEF=FE(33.0=100)
A:
NA
C2:
PF0061714=RasGEF=FE(15.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAAAGTCGTGCCCTACCACTG
R:
GAGCAGAGAGGATGGCGTAGA
Band lengths:
257-446
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types