Special

MmuINT0179265 @ mm9

Intron Retention

Gene
ENSMUSG00000033961 | Zfp446
Description
zinc finger protein 446 [Source:MGI Symbol;Acc:MGI:2442185]
Coordinates
chr7:13564347-13564995:+
Coord C1 exon
chr7:13564347-13564727
Coord A exon
chr7:13564728-13564799
Coord C2 exon
chr7:13564800-13564995
Length
72 bp
Sequences
Splice sites
5' ss Seq
TGGGTGAGT
5' ss Score
8.73
3' ss Seq
TGTGACATGATTCTCTTCAGATC
3' ss Score
7.99
Exon sequences
Seq C1 exon
GTCCCTCTGTCACCTCCAAAAAGACCCTTCAAAACAAGAATGCCATCTCCTCGGGGTACCCCACACCTATCCCTCAGAGACTCCAGGACCACCCTTGAGGAGCCTGAGGCTGCACGACTCCGTTTCCGGGGTTTCTGCTATGAGGAGGTTGAGGGACCCCGAGAGGCACTGGCCCAGCTTCGAGAGTTGTGTCACCAGTGGCTACAGCCTGAGTCATCCTCCAAAGAACACATAATAGAGTTGCTGATTTTGGAACAGTTCCTGGGTGTGTTGCCCCCTGAGATCCAGGCCTGGGTTCGAGGGCAGCGACCAGGAAGCCCCGAGGAGGCCGCAGTTCTTGTTGAGGGGCTGCAGCATGATCCTGGGCAGCTGCTGGGCTGG
Seq A exon
GTGAGTGAAATGACATTAGCTTCTTAGAAGGACAGATCGAAGGGAGAGCCCCTGTGACATGATTCTCTTCAG
Seq C2 exon
ATCACAGCCCACATCCTGAAGCCAAAGATGCTTCCTCTAGTCCAGAAGGAGTCCTCAGGGAGTCACCACATCTCAGCAGCAACGGAGTCCTCCAAGGCAGGCCTTGCGGAGGCACCGCTGAAGGCCGGGATAGATCGATCTACCCAGATCAGTTGCAGTGTGAAGGAGGAAGTCAGTGCTGATGGGCAGGAGATGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000033961-Zfp446:NM_001168562:2
Average complexity
IR-C
Mappability confidence:
NA
Protein Impact





	

	
ORF disruption upon sequence inclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.313 A=NA C2=0.473
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0202312=SCAN=WD(100=73.2)

							
A:
NA

							
C2:
NO

						

					

				








    
Main Inclusion Isoform:
NA





     
                   









    
Main Skipping Isoform:
ENSMUSP00000039073





     
      









    
Other Inclusion Isoforms:
NA
          









    
Other Skipping Isoforms:
ENSMUSP00000104175, ENSMUSP00000104176, ENSMUSP00000104178
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr19:58477561-58477636 
ALTERNATIVE
HsaINT0187285
(ZNF446)
Human
(hg19)
chr19:58988928-58989003 
ALTERNATIVE
HsaINT0187285
(ZNF446)
Rat
(rn6)
chr1:65600433-65600504 
ALTERNATIVE
RnoINT0000339
(AABR07002065.2)
Cow
(bosTau6)
chr18:65907938-65908012 
LOW PSI
BtaINT0165510
(ZNF446)
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
TGAGATCCAGGCCTGGGTTC

				
R: 
GCTGCTGAGATGTGGTGACTC

				
Band lengths: 
183-255

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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