RnoEX0081774 @ rn6
Exon Skipping
Gene
ENSRNOG00000022922 | Slc25a12
Description
solute carrier family 25 member 12 [Source:RGD Symbol;Acc:1305181]
Coordinates
chr3:57936954-57946323:-
Coord C1 exon
chr3:57946208-57946323
Coord A exon
chr3:57941165-57941268
Coord C2 exon
chr3:57936954-57937093
Length
104 bp
Sequences
Splice sites
3' ss Seq
GATGGCTTGTATACCTACAGGAG
3' ss Score
6.67
5' ss Seq
GAGGTAATT
5' ss Score
8.88
Exon sequences
Seq C1 exon
GTTGATCTCCTATCAAGAGTTTTTGGCATTTGAATCTGTTCTATGTGCTCCAGATTCCATGTTCATAGTGGCTTTCCAACTGTTTGACAAGAGTGGAAATGGAGAGGTGACATTTG
Seq A exon
GAGGAGTTCTCTGGCCACAGCGTTGGTACAGCAGCTCCTTCCAGGCTCATTCATGGAGCAGGCATGGTGGCAGTCTTGGAGAAGAGTGCCAGCCCCGGTCAGAG
Seq C2 exon
AAAATGTCAAGGAGATTTTCGGACAGACTATTATCCATCACCACATCCCCTTTAACTGGGACTGCGAGTTCATCCGGCTGCACTTTGGGCACAACCGCAAGAAGCACCTGAACTATGTGGAATTCACTCAGTTCCTCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000022922_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
In the CDS, with uncertain impact
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.286 C2=0.000
Domain overlap (PFAM):
C1:
PF134991=EF-hand_7=PD(18.5=25.0),PF0003627=EF-hand_1=PU(65.5=47.5)
A:
NO
C2:
PF0003627=EF-hand_1=PD(31.0=19.1)
Main Inclusion Isoform:
ENSRNOT00000036728fB13542
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCTCCTATCAAGAGTTTTTGGCA
R:
GGAGGAACTGAGTGAATTCCACA
Band lengths:
249-353
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with negative fitness/survivability impact when depleted: HeLa No Change at 14 days (FC=0.823, FDR=0.001), PC9 Depleted at 14 days (FC=0.543, FDR=0.000), Late Xenograft Depleted (FC=0.683, FDR=0.000).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]