BtaEX0032848 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000016762 | SLC25A12
Description
Bos taurus solute carrier family 25 (mitochondrial carrier, Aralar), member 12 (SLC25A12), mRNA. [Source:RefSeq mRNA;Acc:NM_001101194]
Coordinates
chr2:24715049-24723421:+
Coord C1 exon
chr2:24715049-24715164
Coord A exon
chr2:24719694-24719797
Coord C2 exon
chr2:24723282-24723421
Length
104 bp
Sequences
Splice sites
3' ss Seq
GATGGCTTGTATACCTACAGGAG
3' ss Score
6.67
5' ss Seq
GAGGTAATT
5' ss Score
8.88
Exon sequences
Seq C1 exon
GTTGATCTCCTATCAAGAGTTTTTGGCATTTGAATCTGTATTATGTGCTCCAGATTCCATGTTCATAGTGGCTTTCCAATTGTTTGACAAGAGTGGAAATGGAGAGGTGACATTTG
Seq A exon
GAGGAGTTCTCTGGCCACACCGTTGGTACAGCAGCTCCTTCCAGGCTCATTCATGGAACAGGCATGGTGGCAGTCTTGGAGAAGAGTGCCAGCCCCGGTCAGAG
Seq C2 exon
AAAATGTCAAAGAAATTTTTGGACAGACTACTATTCACCATCATATCCCTTTTAATTGGGACTGTGAATTTATCCGGCTACATTTTGGGCATAATCGGAAGAAGCACCTTAACTACACAGAATTCACACAGTTTCTTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000016762_MULTIEX1-1/2=C1-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
In the CDS, with uncertain impact
No structure available
Features
Disorder rate (Iupred):
C1=0.004 A=0.143 C2=0.000
Domain overlap (PFAM):
C1:
PF134991=EF-hand_7=PD(14.5=25.0),PF0003627=EF-hand_1=PU(65.5=47.5)
A:
PF0003627=EF-hand_1=PD(0.1=0.0)
C2:
PF0003627=EF-hand_1=PD(31.0=19.1),PF134991=EF-hand_7=PU(42.9=51.1)
Main Inclusion Isoform:
ENSBTAT00000022292fB6429
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGATCTCCTATCAAGAGTTTTTGGCA
R:
TGTGAATTCTGTGTAGTTAAGGTGCT
Band lengths:
242-346
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with negative fitness/survivability impact when depleted: HeLa No Change at 14 days (FC=0.823, FDR=0.001), PC9 Depleted at 14 days (FC=0.543, FDR=0.000), Late Xenograft Depleted (FC=0.683, FDR=0.000).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development