MmuEX0042827 @ mm9
Exon Skipping
Gene
ENSMUSG00000027010 | Slc25a12
Description
solute carrier family 25 (mitochondrial carrier, Aralar), member 12 [Source:MGI Symbol;Acc:MGI:1926080]
Coordinates
chr2:71162033-71171777:-
Coord C1 exon
chr2:71171662-71171777
Coord A exon
chr2:71166491-71166594
Coord C2 exon
chr2:71162033-71162172
Length
104 bp
Sequences
Splice sites
3' ss Seq
GATGGCTTGTATACCTACAGGAG
3' ss Score
6.67
5' ss Seq
GAGGTAATT
5' ss Score
8.88
Exon sequences
Seq C1 exon
GTTGATCTCCTATCAAGAGTTTTTGGCATTTGAATCTGTTCTATGTGCTCCAGATTCCATGTTCATAGTGGCTTTCCAACTGTTTGACAAGAGCGGAAATGGAGAGGTGACATTTG
Seq A exon
GAGGAGTTCTCTGGCCACAGCGTTGGTACAGCAGCTCCTTCAAGGCTCAGTCACGGAGCGGGCACGGTGGCAGGCTTGGAGAAGAGTGCCAGCCCCGGTCAGAG
Seq C2 exon
AAAATGTCAAGGAGATTTTTGGGCAGACTATTATCCATCACCACATCCCTTTTAACTGGGACTGCGAGTTCATCCGGCTGCACTTTGGGCACAACCGCAAGAAGCACCTGAACTATGTGGAATTCACTCAGTTCCTCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000027010_MULTIEX1-3/3=2-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
In the CDS, with uncertain impact
Show structural model
Features
Disorder rate (Iupred):
C1=0.000 A=0.286 C2=0.000
Domain overlap (PFAM):
C1:
PF134991=EF-hand_7=PD(14.5=25.0),PF0003627=EF-hand_1=PU(65.5=47.5)
A:
PF0003627=EF-hand_1=PD(0.1=0.0)
C2:
PF0003627=EF-hand_1=PD(31.0=19.1)
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCTCCTATCAAGAGTTTTTGGCA
R:
GGAGGAACTGAGTGAATTCCACA
Band lengths:
249-353
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with negative fitness/survivability impact when depleted: HeLa No Change at 14 days (FC=0.823, FDR=0.001), PC9 Depleted at 14 days (FC=0.543, FDR=0.000), Late Xenograft Depleted (FC=0.683, FDR=0.000).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types