RnoEX6003460 @ rn6
Exon Skipping
Gene
ENSRNOG00000025167 | Sema4b
Description
semaphorin 4B [Source:RGD Symbol;Acc:1307872]
Coordinates
chr1:142008152-142008988:+
Coord C1 exon
chr1:142008152-142008362
Coord A exon
chr1:142008472-142008587
Coord C2 exon
chr1:142008822-142008988
Length
116 bp
Sequences
Splice sites
3' ss Seq
AGTCATGTTCCTGATTCCAGGTG
3' ss Score
7.04
5' ss Seq
GGGGTGAGT
5' ss Score
7.93
Exon sequences
Seq C1 exon
TGTATCACCAACAGTGCCCGGGAACGGAAGATCAACTCGTCGCTGCAGCTCCCGGACCGAGTGCTGAACTTCCTCAAGGATCACTTCTTGATGGATGGGCAGGTCCGCAGCCGCCTGCTGCTGCTCCAGCCACAAGCCCGCTACCAGCGTGTGGCCGTGCACCGAGTGCCTGGCCTGCACAGCACGTATGATGTACTATTTCTGGGCACTG
Seq A exon
GTGATGGTCGCCTGCACAAGGCAGTGACCCTGGACTCCAGAGTGCACATCATTGAGGAGCTGCAGGTCTTCCCTCAAGGACAGCCTGTGCAGAACCTGCTCTTGGACAGCCATGGG
Seq C2 exon
GGACTGTTGTATGCCTCCTCCCATTCTGGGGTAGTGCAAGTGCCCGTAGCCAACTGCAGCCTGTACCCAACCTGTGGAGACTGCCTCCTGGCTCGAGACCCTTACTGCGCCTGGAACGGCTCCACCTGCAAGCTCCTTAGTCTCTACCAGCCTGATCTGGCCTCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000025167-'12-14,'12-13,13-14=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.056 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(16.0=100)
A:
PF0140314=Sema=FE(8.7=100)
C2:
PF0143720=PSI=PU(64.4=67.9)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAGATCAACTCGTCGCTGCAG
R:
GTACAGGCTGCAGTTGGCTAC
Band lengths:
250-366
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]