RnoEX6034322 @ rn6
Exon Skipping
Gene
ENSRNOG00000014084 | Sp1
Description
Sp1 transcription factor [Source:RGD Symbol;Acc:3738]
Coordinates
chr7:144015560-144032682:+
Coord C1 exon
chr7:144015560-144015717
Coord A exon
chr7:144015999-144017511
Coord C2 exon
chr7:144032514-144032682
Length
1513 bp
Sequences
Splice sites
3' ss Seq
CTTTCCTCTCTTCTGGCCAGGAA
3' ss Score
9.3
5' ss Seq
CAGGTAAGA
5' ss Score
10.77
Exon sequences
Seq C1 exon
ACCAAGATCACTCAATGGATGAAGTGACAGCTGTGAAGATTGAAAAAGGTGTTGGTGGCAATAACGGGGGTAGCGGGAACGGTGGTGGTGCCGCCTTTTCTCAGACTCGAAGCAGCAGCACAGGCAGTAGCAGCAGCAGTGGTGGCGGAGGAGGGCAG
Seq A exon
GAATCCCAGCCATCTCCTTTGGCTCTGCTGGCAGCAACCTGCAGCAGAATTGAGTCACCCAATGAGAACAGCAACAACTCCCAGGGTCCGAGTCAGTCAGGGGGCACAGGTGAACTTGACCTCACAGCCACACAACTTTCACAGGGTGCCAATGGCTGGCAGATCATCTCTTCCTCCTCTGGGGCTACCCCTACCTCAAAGGAACAGAGTGGCAACAGTACCAATGGCAGCAATGGCAGTGAGTCTTCCAAGAACCGCACAGTCTCTGGTGGGCAGTATGTTGTGGCTGCTACCCCCAACTTACAGAACCAGCAAGTTCTGACAGGTCTACCTGGAGTAATGCCTAATATTCAGTATCAAGTAATCCCACAGTTCCAGACTGTTGATGGGCAACAGCTGCAGTTTGCTGCCACTGGGGCCCAAGTGCAGCAGGATGGTTCTGGTCAAATACAGATCATACCAGGTGCAAATCAACAGATCATCACAAATAGAGGAAGTGGGGGCAACATTATTGCTGCTATGCCAAATCTACTCCAGCAAGCTGTCCCCCTCCAAGGCCTTGCTAATAATGTGCTCTCAGGACAGACTCAGTATGTGACCAATGTACCAGTGGCCCTGAATGGGAACATCACCTTGCTACCTGTCAACAGCGTTTCTGCAGCTACCCTGACTCCCAGCTCTCAGGCAGGCACGATCAGCAGCTCTGGATCCCAGGAGAGTGGCTCACAGCCTGTCACCTCAGGGACTGCCATCAGTTCTGCCAGCTTGGTGTCATCACAAGCCAGTTCCAGCTCCTTTTTCACCAATGCCAACAGCTATTCAACAACTACCACCACCAGCAACATGGGAATTATGAACTTTACCAGCAGCGGATCATCAGGGACTAGTTCTCAAGGCCAGACATCCCAGAGAGTTGGTGGGCTGCAAGGGTCTGATTCTCTAAACATCCAGCAGAACCAGACATCAGGAGGATCACTGCAAGGAAGTCAGCAAAAAGAGGGAGAGCAAAGTCAGCAGACACAGCAACAACAAATTCTTATTCAGCCTCAGCTAGTTCAAGGTGGACAAGCTCTTCAGGCCCTCCAAGCAGCACCATTGTCAGGACAGACCTTCACAACTCAAGCTATTTCCCAGGAAACCCTCCAGAACCTCCAGCTTCAGGCTGTTCAAAACTCTGGCCCCATCATCATTCGGACACCAACCGTGGGGCCCAATGGACAGGTCAGTTGGCAGACCCTTCAGCTGCAGAACCTTCAAGTTCAGAACCCACAAGCCCAGACAATCACCTTGGCCCCTATGCAGGGTGTTTCCTTGGGGCAGACTAGCAGCAGCAATACCACCCTTACACCCATTGCCTCAGCTGCCTCCATTCCTGCTGGCACAGTCACTGTGAATGCTGCTCAACTGTCCTCCATGCCAGGCCTCCAGACCATTAACCTCAGTGCATTGGGTACTTCAGGGATCCAGGTGCACCAGCTTCCAGGCCTGCCTTTGGCTATAGCAAACACCCCAG
Seq C2 exon
GTGATCATGGAGCTCAACTTGGCCTTCATGGACCTGGCGGTGATGGAATACATGATGAGACAGCAGGTGGAGAAGAAGGAGAGAACAGCCCTGATCCCCAACCCCAAGCTGGTCGCAGGACTCGGCGGGAAGCATGTACATGCCCCTATTGTAAAGACAGTGAGGGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000014084-'5-6,'5-1,8-6=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=0.545 C2=0.719
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Mouse
(mm9)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACCAAGATCACTCAATGGATGAAGT
R:
TCCCCTCACTGTCTTTACAATAGG
Band lengths:
326-1839
Functional annotations
There are 1 annotated functions for this event
PMID: 11904305
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: classical fluorescence spectroscopy, mutation analysis, protein kinase assay. ELM ID: ELMI001445; ELM sequence: IIRTPTV; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]