RnoINT0049223 @ rn6
Intron Retention
Gene
ENSRNOG00000000542 | Dnah8
Description
dynein, axonemal, heavy chain 8 [Source:RGD Symbol;Acc:619986]
Coordinates
chr20:9467861-9468171:+
Coord C1 exon
chr20:9467861-9467883
Coord A exon
chr20:9467884-9468015
Coord C2 exon
chr20:9468016-9468171
Length
132 bp
Sequences
Splice sites
5' ss Seq
CCCCCCGAC
5' ss Score
-39.1
3' ss Seq
CCCCATTTCCCGCTCTTCAGATT
3' ss Score
8.61
Exon sequences
Seq C1 exon
GTTGGTTTGTCCTGCCCCCCCCC
Seq A exon
CCCGACACCGACTAGACACCTCAAATGGAAACACATATTTAAATTATTGCCATTTAACGTTATAGGCTCTCAATGACCAACGGTCACATTCCTGTTCTTTAAGCGAACATCCCCCCATTTCCCGCTCTTCAG
Seq C2 exon
ATTGGCGAGTGGGGCCTGCAGGGACTCCCTGGAGATGACTTGTCAATTCAGAATGGCATTATTGTGACAAAGGCTACCAGATACCCTCTGCTTATTGATCCCCAGACCCAGGGGAAAACGTGGATTAAATCGAAGGAGAAAGAAAACGACTTGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000000542:ENSRNOT00000000651:90
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.058
Domain overlap (PFAM):
C1:
PF127812=AAA_9=PU(0.4=12.5)
A:
NA
C2:
PF127812=AAA_9=FE(22.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTTGGTTTGTCCTGCCCCC
R:
TGCAAGTCGTTTTCTTTCTCCT
Band lengths:
178-310
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]